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| Determination of eight ginsenosides in Ginseng extract by UPLC |
| Hits 1948 Download times 1558 Received:April 30, 2014 |
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| DOI
10.11656/j.issn.1672-1519.2014.10.13 |
| Key Words
Ginseng extract;ginsenoside;UPLC;assay |
| Author Name | Affiliation | E-mail | | LI Wei | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Tianjin Zhong Yi Pharmaceutical Co., Ltd., Tianjin 300193, China | | | JI Li-na | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | SONG Xin-bo | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Tianjin Zhong Yi Pharmaceutical Co., Ltd., Tianjin 300193, China | songxinbo@tjutcmeducn |
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| Abstract
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| [Objective] To develop a ultra-high performance liquid chromatography method for simultaneously determination of eight ginsenosides in Ginseng extract. [Methods] The analysis was performed on a Waters Acqutity UPLC system eluted with mobile phases of acetonitrile and water in gradient mode. The column was Acqutity UPLC®BEH C18 (1.7 μm, 2.1 mm×50 mm). The flow rate was 0.6 mL/min. The column temperature was set at 40 ℃ and the detection wavelength was set at 203 nm. [Results] Good linearity was obtained in the range of 0.009 7~0.291 0 μg, 0.008 5~0.255 0 μg, 0.007 5~0.300 0 μg, 0.010 4~0.416 0 μg, 0.010 7~0.428 0 μg, 0.007 4~0.296 0 μg, 0.004 2~0.168 0 μg and 0.009 1~0.364 0 μg for ginsenoside Rg1, Re, Rf, Rb1, Rc, Rb2, Rb3 and Rd respectively. The method recoveries of eight compounds were 101.4%, 101.1%, 100.1%, 99.8%, 99.3%, 98.8%, 98.8% and 100.5% respectively. [Conclusion] The established method can rapidly receive an accurate and reproducible result used for controlling the quality of Ginseng extract. |
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