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| LC-MS/MS determination of caffeic acid, ferulic acid acid, and isoferulic acid in Rhizoma Cimicifugae |
| Hits 1604 Download times 1610 Received:September 09, 2014 |
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| DOI
10.11656/j.issn.1672-1519.2014.11.13 |
| Key Words
LC-MS/MS;Rhizoma Cimicifugae;caffeic acid;ferulic acid;isoferulic acid |
| Author Name | Affiliation | | WANG Xiao-ming | Tianjin University of Traditional Chinese Medicine, Modern Chinese Medicine-Province and Ministry Coestablished State Key Laboratory Cultivation Base, Tianjin Key Laboratory Tianjin 300193, China | | ZHANG Fan | Tianjin University of Traditional Chinese Medicine, Modern Chinese Medicine-Province and Ministry Coestablished State Key Laboratory Cultivation Base, Tianjin Key Laboratory Tianjin 300193, China | | LIU Ying | Tianjin University of Traditional Chinese Medicine, Modern Chinese Medicine-Province and Ministry Coestablished State Key Laboratory Cultivation Base, Tianjin Key Laboratory Tianjin 300193, China
Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin, 300457 | | ZHANG Na | Tianjin University of Traditional Chinese Medicine, Modern Chinese Medicine-Province and Ministry Coestablished State Key Laboratory Cultivation Base, Tianjin Key Laboratory Tianjin 300193, China
Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin, 300457 |
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| Abstract
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| [Objective] To develop a LC-MS/MS method for simultaneous determination of caffeic acid, ferulic acid and isoferulic acid in the Rhizoma Cimicifugae. [Methods] Chromatographic separation was achieved with gradient elution by a ACQUITY UPLC® BEH C18 coloum (2.1 mm×100 mm,1.7 μm) hyphenated to a mass spectrometer system was operated under the MRM mode with electrospray negative ionization. The mobile phase was acetonitrile-0.1% formic acid aqueous and the flow rate was 0.3 mL/min. [Results] Caffeic acid, ferulic acid and isoferulic acid showed good linearity in the range of 5~100 ng/mL, 10~200 ng/mL, 50~1 000 ng/mL. The lower limit of quantification of the 3 essential compounds was 5, 10 and 50 ng/mL respectively. The average recoveries of the method were in the range of 97.83%~98.40%. [Conclusion] The method is fast, sensitive and reproducible. It can be used for the simultaneous determination of caffeic acid, ferulic acid and isoferulic acid in the Rhizoma Cimicifuga. |
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