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| Influence of Zhuanggu Guanjie pill on liver microsome cytochrome P450 in rats |
| Hits 1486 Download times 1516 Received:May 23, 2014 |
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| DOI
10.11656/j.issn.1672-1519.2014.11.14 |
| Key Words
Zhuanggu Guanjie pill;cytochrome P450;CYP1A2;CYP2E1;CYP2C19;CYP3A |
| Author Name | Affiliation | | ZHOU Kun | Key Laboratory of Traditional Chinese Medical Formulae, Ministry of Education, Institute of Traditional Chinese Medicine, Tianjin University of TCM, Tianjin 300193, China | | ZHU Tao-tao | Key Laboratory of Traditional Chinese Medical Formulae, Ministry of Education, Institute of Traditional Chinese Medicine, Tianjin University of TCM, Tianjin 300193, China | | ZHANG Yue | Key Laboratory of Traditional Chinese Medical Formulae, Ministry of Education, Institute of Traditional Chinese Medicine, Tianjin University of TCM, Tianjin 300193, China | | DAI Zhi | R&D Center of Sanjiu Medical & Pharmacutical Co. Ltd, Shenzhen 518110, China |
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| Abstract
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| [Objective] To investigate the influence of Zhuanggu Guanjie pill (ZGGJ) on liver microsome cytochrome P450 in rats. [Methods] Rats were administered intragastrically with ZGGJ and their two new technologic products(crude drug, 2.1 g/kg)once a day for 7 days. The 24 hours after the last administration, rats were sacrificed and liver microsomes were prepared by calcium sedimentation. CYP3A, CYP1A2, CYP2C19, CYP2E1 activity of liver microsomes, including dapsone, phenacetin, omeprazole and chlorzoxazone were detected by cocktail probe in vitro. [Results] Cocktail probes were metabolized in liver microsomes system for 1 h. Chlorzoxazone concentration of administrated groups, including ZGGJ and their two new technologic products groups was significantly higher than control group. There was no significant difference of omeprazole, phenacetin, dapsone concentrations between administrated groups and the control group. [Conclusion] ZGGJ can inhibit CYP2E1 activity, but has no significant effect on CYP1A2, CYP3A and CYP2C19 in rats. |
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