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| Experimental searches for Yigan Jiangzhi Fang on peroxide damage |
| Hits 1721 Download times 1453 Received:May 25, 2014 |
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| DOI
10.11656/j.issn.1672-1519.2014.12.13 |
| Key Words
Yigan Jiangzhi Fang;alcoholic fatty liver disease;oxidative stress;pretreatment;pathology |
| Author Name | Affiliation | E-mail | | ZHAO Yuan-hong | Department of Oncology, The First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | ZUO Xiao-na | Graduate School of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | HAN Su-heng | Graduate School of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | XU Yu | Graduate School of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | JIA Ying-jie | Department of Oncology, The First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | LI Zheng | Department of Oncology, The First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Manufacturing Laboratory, The First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300385, China | lizheng@shitian.com |
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| Abstract
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| [Objective] To explore intervention effect of oxidative stress effect for AFLD rats at different times, to analyze antioxygenation of YGJZF to AFLD rats and surgery its possible mechanisms. [Methods] The 97 SD rats were randomized into model group 22 and YGJZF group 75, which were divided into six groups. The model rats were induced by gradient ethanol gavage and high fat feeding method. Alcohol by gavage in half an hour, rats in YGJZF groups were given equivalent and high dose[13.95(g/kg·d)and 27.9(g/kg·d)]of YGJZF since the third, sixth and ninth week respectively, and in model groups normal saline(1.5 mL/kg), until the end of 12th week, at the end of third, sixth and ninth week, the serum values of ALT, AST, TC, TG, 8-iso-PGF2α, and hepatic tissue' change of MDA, XOD and pathological. [Results] Compared with corresponding model groups, at the end of the 6th week, in 3w-Ⅱ group the level of 8-iso-PGF2α, MDA and XOD reduced obviously(P<0.05). At the end of the 9th and 12th week, the level of 8-iso-PGF2α、MDA and XOD reduced obviously(P<0.05), particularly in II group. At the end of 12th week, the results in the 3 w group were the best, and in the 9 w group were the worst, the difference between 3w-I, 9w-I and 6w-I in TG, 8-iso-PGF2α, MDA was remarkable(P<0.05); each index in YGJZF-II group was lower in YGJZF-I group, especially the value of 8-iso-PGF2α in 3w group; 8-iso-PGF2α and MDA in 6w group; XOD in 9w group(P<0.05). At the end of the 6th, 9th and 12th week, compared with model groups, the degree of hepatic fatty change alleviated to some extent particularly in II group; and at the end of the 12th week, the change in 3 w group was beater than in 6w group, in the same between the 6w group and 9w group. [Conclusion] This article showed that YGJZF played a role in reducing SD rats' oxidative stress injury caused by alcohol in different periods of intervention, especially when the earlier intervention was done, and restrained pathological injure of AFLD rats presenting time and concentration dependence to some extent, which provided the experimental basis for the chooses of medication and time in the prevention and control of AFLD in clinic. |
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