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| Effect of Fushen Jiangzhuo formula on anti-fibrosis factors of HGF and BMP-7 in rats with renal tubulointerstitial lesion |
| Hits 1623 Download times 1285 Received:August 20, 2014 |
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| DOI
10.11656/j.issn.1672-1519.2014.12.14 |
| Key Words
Fushen Jiangzhuo formula;renal tubulointerstitial lesion;hepatocyte growth factor;bone morphogenetic protein-7 |
| Author Name | Affiliation | E-mail | | LI Chun-yu | International Medical School, Tianjin Medical University, Tianjin 300070, China | | | WEI Xiao-lu | International Medical School, Tianjin Medical University, Tianjin 300070, China | | | SU Wei-lian | International Medical School, Tianjin Medical University, Tianjin 300070, China | | | LI Guo-xia | International Medical School, Tianjin Medical University, Tianjin 300070, China | liguoxia96@163.com |
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| Abstract
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| [Objective] To study the protective effects and mechanism of Fushen Jiangzhuo formula (FSJZ) on renal tubulointerstitial lesion in rats. [Methods] The Mesangial proliferative glomerulonephritis (MsPGN) in rats was produced and through extending the time of the modeling to 20th weeks the model was naturally developed to tubulointerstitial lesion. The rats were divided into 4 groups, including Chinese medicine group, Western medicine positive control group, model group and blank group. FSJZ formula was given to the rats of Chinese medicine group and benazepril was given to Western medicine group, while the same volume of saline was given to the animals in model group and blank group for 20 weeks. We isolated the kidney tissue of the rats at the end of 12, 16, 20 week and observed the effect of FSJZ on anti fibrosis factors of HGF and BMP-7 in renal tissue by real-time reverse transcription-polymerase chain reaction and immunohistochemistry method. [Results] The anti-fibrosis factors of HGF and BMP-7 changed in renal tubulointerstitial lesion in rats. Renal tubulointerstitial lesion could significantly down-regulate the expressions of HGF and BMP-7, while FSJZ could significantly up-regulate the expressions of HGF and BMP-7 and antagonize these effects caused by renal tubulointerstitial lesion. [Conclusion] FSJZ has distinct effects of protecting pathological renal interstitial fibroblasts. The mechanism may be related with the regulating effect of FSJZ on mRNA and protein expression of HGF and BMP-7. |
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