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| Experimental study on the effect of oligo-peptide I-C-F-6 in Carapax Trionycis against chronic alcoholic liver injury in mice |
| Hits 1779 Download times 1748 Received:September 08, 2014 |
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| DOI
10.11656/j.issn.1672-1519.2015.01.08 |
| Key Words
oligo-peptide I-C-F-6;chronic alcoholic liver injury;protective effect |
| Author Name | Affiliation | E-mail | | WANG Mi-na | School of Preclinical Medicine, Beijing University of Chinese Medcine, Beijing 100029, China | | | XU Shi-xun | School of Chinese Pharmacy, Beijing University of Chinese Medcine, Beijing 100102, China | | | LIN Jin-xuan | School of Preclinical Medicine, Beijing University of Chinese Medcine, Beijing 100029, China | | | REN Li-wei | School of Preclinical Medicine, Beijing University of Chinese Medcine, Beijing 100029, China | | | LEI Hai-min | School of Chinese Pharmacy, Beijing University of Chinese Medcine, Beijing 100102, China | | | ZHANG Yu-zhong | School of Preclinical Medicine, Beijing University of Chinese Medcine, Beijing 100029, China | zyz100102@126.com |
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| Abstract
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| [Objective] To study the protective effect of oligo-peptide I-C-F-6 against chronic alcohol hepatic fibrosis in mice and to explore its possible mechanisms. [Methods] The ICR mice were randomly divided into five groups: normal control group, model group, oligo-peptide low dose group (0.12 mg/kg), oligo-peptide high dose group (0.24 mg/kg), positive drug group (240.00 mg/kg). Mice in oligo-peptide groups were administered oligo-peptide' saline solution (0.12 mL/kg) once a day, while mice in normal group and model group were given saline solution (0.12 mL/kg); positive drug group was given reduced-glutathione (0.12 mL/kg) by intragastric administration once a day. At the same time, in addition to the normal control group, the rest of the four groups were given 40% alcohol once a day, for 16 weeks. After 16 weeks, they were sacrificed for collecting the blood samples and liver tissue samples. At last, the serum alanine aminotransferase (ALT), aspertate aminotransferase (AST) and alkaline phosphatase (AKP) level and superoxide dismutase (SOD) in liver tissue and malondialdehyde (MDA) and monoamne oxidase (MAO) activity content were determined respectively. The changes of pathohisology of mice were observed. [Results] ALT, AST, AKP in mice, MAO, MDA content was significantly higher in chronic alcoholic liver injury model group. Compared with model group, the serum concentrations of ALT, AST, AKP and the liver tissue content of MDA and MAO were significantly decreased in treatment groups (P<0.05); the activity of SOD in the liver tissue in treatment groups were also significantly increased (P<0.05). Chronic alcoholic liver injury was significantly improved in treatment groups as compared with the model group. [Conclusion] Oligo-peptide I-C-F-6 has significant therapeutic effect on chronic alcoholic liver injury in mice. Its mechanism may be related to the inhibition of lipid peroxidation, scavenging free radicals and increasing the vitality of SOD. |
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