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| Effect of Wutian Baogan Ye on expression of MMP-2 and TIMP-2 mRNA of liver tissue in rats with alcohol hepatic fibrosis |
| Hits 1686 Download times 1396 Received:August 28, 2014 |
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| DOI
10.11656/j.issn.1672-1519.2015.01.09 |
| Key Words
Wutian Baogan Ye;alcohol hepatic fibrosis;MMP-2;TIMP-2 |
| Author Name | Affiliation | E-mail | | SU Jin-ling | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | JIANG Xi-Juan | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | ZHANG Yun-sha | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | GUO Mao-juan | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | FAN Ying-chang | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | iamcloud@yahoo.cn |
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| Abstract
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| [Objective] To investigate the effect of Wutian Baogan Ye on the prevention and treatment of rats with alcohol hepatic fibrosis(AHF)in liver tissue and the possible mechanism. We studied the expression of collagen type IV,MMP-2 and TIMP-2 in liver tissue of rats with AHF. [Methods] The 100 Wistar rats were assigned to six groups randomly: normal group (10), Wutianbaoganye low dose group(18), middle dose group(18), high dose group(18), model control group(18), positive drug group(18). Rat model of AHF was induced by feeding stomach with the mixture of liquor, corn oil and pyrazol. The 12 weeks later, the expression of collagen type IV, MMP-2 and TIMP-2 in liver tissue of rats were observed. [Results] Compared with normal rats, the expression of collagen type IV of rats in all group was increased. Compared with model rats, Wutian Baogan Ye (especially for Wutian Baogan Ye middle, and high dose) and positive drug could decrease the expression of collagen type IV. Compared with normal rats, the expression of MMP-2 and TIMP-2 was increased in model rats, but the TIMP-2 was increased more obviously. Compared with the model rats, the expression of MMP-2 was increased, and the expression of TIMP-2 was decreased in the rats given drug (especially for Wutian Baogan Ye middle and high dose). [Conclusion] Wutian Baogan Ye has an active effect on inhibiting alcohol hepatic fibrosis in rats. Wutian Baogan Ye can increase the expression of MMP-2 and decrease the expression of TIMP-2. |
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