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| Effect of Fuke Guanchang II on secretion of estrogen and prostaglandin E2 from isolated cultured ectopic stromal cells |
| Hits 2369 Download times 1924 Received:September 30, 2014 |
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| DOI
10.11656/j.issn.1672-1519.2015.01.10 |
| Key Words
endometriosis;estrogen;prostaglandin E2;Fuke Guanchang;Caco-2 cell model |
| Author Name | Affiliation | E-mail | | DAI Zhi-rong | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | LI Pei-lin | The Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300150, China | peilin@wo.com.cn | | WANG Pan-pan | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | ZHAO Zhi-mei | The Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300150, China | | | LI Jie | The Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300150, China | | | WANG Jian-ling | The Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300150, China | | | ZHANG Shu-ying | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | |
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| Abstract
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| [Objective] To investigate the effects of Fuke GuanchangⅡrecipe (enema II for patients in gynecotokology) on secretion of E2 and prostag landin E2(PGE2) from ectopic endometrial cells. [Methods] Using the mono-layer model of Caco-2 cells as a tool, miming the enema method used in traditional Chinese medicine in human body, the endometrial stromal cells and Caco-2 cells were co-cultured together. The Fuke Guanchang II and other subjects factors were stimulated on the endometriosis ectopic endometrial cells through Caco-2 cell monolayer model. After 24 hours, the level of PGE2 and E2 secreted from endometrial cells was measured. [Results] The Fuke Guanchang II could reduce the E2 and PGE2 content, secreted by the ectopic endometrial cells. [Conclusion] The Fuke Guanchang II can inhibit the development of EMS through reducing the autocrine of E2 in the endometrial stromal cells. |
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