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| Simultaneous determination and quality evaluation of five effective components in Tripterygium tablets |
| Hits 1567 Download times 1760 Received:October 28, 2014 |
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| DOI
10.11656/j.issn.1672-1519.2015.01.11 |
| Key Words
Tripterygium tablet;content determination;LC-MS/MS |
| Author Name | Affiliation | E-mail | | GU Sheng-pan | School of Chinese Pharmacy, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | FU Shu-jun | School of Chinese Pharmacy, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | MUSSA Ally | School of Chinese Pharmacy, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | XUN Ming-jin | School of Chinese Pharmacy, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | SHENG Yong | School of Chinese Pharmacy, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | HE Xin | School of Chinese Pharmacy, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | hexintn@163.com |
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| Abstract
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| [Objective] To establish an LC-MS/MS method for simultaneous determination of five effective componentsof Tripterygium tablets which are: triptolide, celastrol, wilforlide A, wilfordine and triptophenolide. [Methods] The assay was performed on a Zorbax XDB-C18 column(2.1 mm×50 mm, 3.5 μm) and was eluted with a mobile phase which consisted of the methonal of 0.1% formic acid and 40 mmol/L ammonium formate. Atmospheric pressure chemical ionization source with positive ionization mode as well as selected ion monitoring mode was used in the detection. Ion used for quantification was m/z:361.0/105.0,451.2/201.0,455.3/409.7,884.4/856.5, 313.2/225.2, respectively. [Results] There was a good linear relationship over the studied concentration for the five studied components(r >0.999). The recoveries were 105.67%,94.07%,101.90%,99.28%,101.92%, respectively. The RSD corresponded were 2.65%,5.17%,2.68%,2.65%,3.27%. [Conclusion] The LC-MS/MS method developed for simultaneous determination of the five effective components is simple and valid. It can be used for quality evaluation and quality control of Tripterygium tablets. |
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