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| Comparing the antioxidant activity of the in vitro and intestinal absorption of Ginkgo biloba flavonoids |
| Hits 1559 Download times 1378 Received:September 28, 2014 |
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| DOI
10.11656/j.issn.1672-1519.2015.01.12 |
| Key Words
Flavonoids;DPPH radical;intestinal absorption;antioxidant activity |
| Author Name | Affiliation | E-mail | | LIU Na | School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China | | | WANG Wen-ping | School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China | | | KANG Qian | School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China | | | ZHAO Bo-chen | School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China | | | LAN Yi | School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China | | | CHEN Yan-yan | School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China | | | WU Qing | School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China | qwu@vip.sina.com |
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| Abstract
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| [Objective] To compare the antioxidant activity of three main flavonoids aglycone from Ginkgo biloba extract in vitro and intestinal absorption. [Methods] Using DPPH (2, 2-diphenyl-1-picryl-hydrazyl-hydrate) radical method and measuring the antioxidant activity of the drugs in vitro and after intestinal absorption. [Results] DPPH radical scavenging capacity: Its EC50 were 78.6, 203.2, 222.0 μm, respectively. The trend of intestinal absorption was: Quercetin> Isorhamnetin> Kaempferol, its clearance rate decreased compared to the data in vitro; three different ratios of intestinal absorption displayed the DPPH clearance of the extract mixing ratio was higher than the others. [Conclusion] Three monomers in vitro and fluid of intestinal absorption are able to scavenge DPPH radical and the ability is: Quercetin> Isorhamnetin> Kaempferol; the reason of intestinal absorption clearance rate decrease may be the monomers break down in the gut and reasons need to further investigate. The intestinal absorption of antioxidant capacity of monomer mixed by the extract ratio shows higher than other proportions. |
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