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| Determination of molecular weight and weight distribution in Licorice Polysaccharide by HPGPC |
| Hits 1518 Download times 2066 Received:October 10, 2014 |
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| DOI
10.11656/j.issn.1672-1519.2015.01.13 |
| Key Words
high performance gel permeation chromatography;Licorice Polysaccharide;molecular weight;quality control |
| Author Name | Affiliation | E-mail | | ZHAO Ying | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | SONG Xin-bo | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | songxinbo@tjutcm.edu.cn | | ZHANG Li-juan | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | YU Teng-fei | Inner Mongolia Medical College, Hohhot 010000, China | |
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| Abstract
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| [Objective] To establish a method for quality control of Licorice polysaccharide. [Methods] Using high performance gel permeation chromatography (HPGPC) for determining the Molecular Weight (MW) and Molecular Weight Distribution of Licorice Polysaccharide; TOSOH TSK gel G4000 PWXL was used as column. Mobile phase was 0.7% Na2SO4 aqueous solution at 0.8 mL/min, column temperature at 40 ℃, injection volume as 20 μL. Detector was differential refraction detector. [Results] The MW of Licorice Polysaccharides was 8.0×104~1.0×105 with good linearity, precision and reproducibility. [Conclusion] This determination method is simple, rapid and accurate. It provides a reference for the quality control of licorice polysaccharide large-scale production. |
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