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| Determining volatiles fromraw materials of Lianhua Qingwen preparations by HS-SPME-GC/MS |
| Hits 1872 Download times 1784 Received:September 29, 2014 |
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| DOI
10.11656/j.issn.1672-1519.2015.02.12 |
| Key Words
Lianhua Qingwen preparation;SPME;GC/MS;volatile constituent |
| Author Name | Affiliation | E-mail | | JIA Wei-na | Tianjin Modern Chinese Medicine Key Laboratory-Province and Ministry co-established State Key Laboratory Cultivation Base, Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Research and Development Center of Traditional Chinese Medicine, Tianjin International Joint Academy of Biotechnology & Medicine, Tianjin 300457, China | | | WANG Chun-hua | Tianjin Modern Chinese Medicine Key Laboratory-Province and Ministry co-established State Key Laboratory Cultivation Base, Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Research and Development Center of Traditional Chinese Medicine, Tianjin International Joint Academy of Biotechnology & Medicine, Tianjin 300457, China | pharmwch@126.com | | WANG Hong-tao | Shijiazhuang Yiling Pharmaceutical Co., Ltd, Shijiazhuang 050035, China
| | | TIAN Shu-yan | Shijiazhuang Yiling Pharmaceutical Co., Ltd, Shijiazhuang 050035, China
| | | HAN Xue | Tianjin Modern Chinese Medicine Key Laboratory-Province and Ministry co-established State Key Laboratory Cultivation Base, Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Research and Development Center of Traditional Chinese Medicine, Tianjin International Joint Academy of Biotechnology & Medicine, Tianjin 300457, China | | | ZHU Yan | Tianjin Modern Chinese Medicine Key Laboratory-Province and Ministry co-established State Key Laboratory Cultivation Base, Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Research and Development Center of Traditional Chinese Medicine, Tianjin International Joint Academy of Biotechnology & Medicine, Tianjin 300457, China | |
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| Abstract
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| [Objective] To analyze the volatiles from raw materials of Lianhua Qingwen preparations. [Methods] Headspace solid-phase micro-extraction (HS-SPME) technique was employed to extract the volatile compounds from raw materials of Lianhua Qingwen preparations. The volatile components were isolated and identified successfully by gas chromatography/mass spectrometry (GC/MS) and matched with NIST05 mass spectra library to retrieve the unknown chemical components automatically, the relative contents in percentage of each chemical composition were calculated by peak area normalization method. [Results] Fifty-three volatile compounds were temporarily identified, corresponding to 98.30% of total oils. The major volatile component was l-menthol (94.84%), and the other high levels of ingredients were patchouli alcohol (1.101%), β-bulnesene (0.398%), β-gurjunene (0.256%), β-guaiene (0.210%), β-patchoulene (0.171%) and α-patchoulene (0.111%). [Conclusion] The volatile components from raw materials of Lianhua Qingwen preparations can be simply, rapidly and accurately analyzed by HS-SPME-GC/MS method. |
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