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| Optimization orthogonal of percolation extraction process of Radix Angelicae Pubescentis coumarin |
| Hits 1874 Download times 1434 Received:September 23, 2014 |
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| DOI
10.11656/j.issn.1672-1519.2015.02.13 |
| Key Words
Radix Angelicae Pubescentis;percolation;orthogonal test;HPLC |
| Author Name | Affiliation | E-mail | | WANG Chun-peng | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | LUO Qian | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | LI Jin | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | HE Jun | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | MA Wen-fang | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | LIU Er-wei | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | CHANG Yan-xu | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | tcmcyx@126.com |
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| Abstract
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| [Objective] To investigate the best percolation extraction condition of Radix Angelicae Pubescent coumarin. [Methods] L9 (34) orthogonal experimental method was used to study the effects of ethanol concentration, solid-liquid ratio, soaking time on the contents of osthole, columbianetin acetate, columbianetin and columbianadin in Radix Angelicae Pubescent extract. [Results] Ethanol concentration was the main factor of affecting percolation,and the optimum extraction was 75% ethanol,solid-liquid ratio of 1:8,soak 8 hours. [Conclusion] This method could be used as the best percolation extraction process of Radix Angelicae Pubescentis coumarin. |
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