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| Study of N-butanol extract of Potentilla anserina L. in down-regulating expression levels of HIF-1α and ET-1 in hypoxia endothelial cells |
| Hits 1561 Download times 1751 Received:September 24, 2014 |
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| DOI
10.11656/j.issn.1672-1519.2015.03.10 |
| Key Words
Potentilla anserina L.;endothelial cell;hypoxia;hypoxia inducible factor-1;endothelin-1 |
| Author Name | Affiliation | E-mail | | YANG Shuo | Tianjin Medical University, Tianjin 300070, China
Logistics University of Chinese People's Armed Police Forces, Tianjin 300309, China | | | ZHANG Ling | Logistics University of Chinese People's Armed Police Forces, Tianjin 300309, China | | | GONG Hai-ying | Logistics University of Chinese People's Armed Police Forces, Tianjin 300309, China | | | ZHANG Yong-liang | Tianjin Key Laboratory for Prevention and Control of Occupational and Environmental Hazards, Tianjin 300309, China | zhang78127@tom.com | | LI Ling-zhi | Logistics University of Chinese People's Armed Police Forces, Tianjin 300309, China
Tianjin Key Laboratory for Prevention and Control of Occupational and Environmental Hazards, Tianjin 300309, China | 13682196000@163.com | | GAO Hong-sheng | Tianjin Key Laboratory for Prevention and Control of Occupational and Environmental Hazards, Tianjin 300309, China | |
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| Abstract
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| [Objective] To study the protective effect of n-butanol extract of Potentilla anserine L. on EA.hy926 endothelial cells exposed to hypoxia. [Methods] The model of hypoxia injury was established with human umbilical vein endothelial cell line (EA.hy926). The cell death was determined by Trypan blue staining assay. The expression levels of HIF-1α and ET-1 mRNA were detected by reverse transcription-PCR assay. The Western blot and imuunocytochemistry were used for determining HIF-1α protein. The ET-1 protein was detected with radioimmunoassay. [Results] Compared with control group, the survival rate of cell was significantly increased after pretreated with 3, 1.5 and 0.75 g/L of n-butanol extract of Potentilla anserine L. Meanwhile, the mRNAs of HIF-1 and ET-1 and their protein products were significantly decreased. [Conclusion] Potentilla anserina L. could exert its protective effect on endothelial cells during hypoxia injury by modulating expression of target genes through HIF-1α pathway. |
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