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| Mechanisms of Cx43 semi-channel in cerebral ischemia-reperfusion injury rats by acupuncture regulation |
| Hits 1588 Download times 1648 Received:November 16, 2014 |
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| DOI
10.11656/j.issn.1672-1519.2015.04.07 |
| Key Words
acupuncture;ischemia reperfusion;PKC;CK1;PP2B |
| Author Name | Affiliation | E-mail | | WANG Zhan-kui | The Acupuncture and Encephalopathy Center, The Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300150, China | | | LIU Kun | The Acupuncture and Encephalopathy Center, The Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300150, China | | | XIAO Zhen-xin | The Acupuncture and Encephalopathy Center, The Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300150, China | | | YANG Bao-wang | The Acupuncture and Encephalopathy Center, The Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300150, China | | | LIN Cui-ru | The Acupuncture and Encephalopathy Center, The Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300150, China | | | ZHOU Zhen | The Acupuncture and Encephalopathy Center, The Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300150, China | | | WANG Dong-ping | The Acupuncture and Encephalopathy Center, The Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300150, China | | | GAO He | The Acupuncture and Encephalopathy Center, The Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300150, China | | | GUO Jia-kui | The Acupuncture and Encephalopathy Center, The Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300150, China | guojiakui@eyou.com |
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| Abstract
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| [Objective] To investigate the dynamic changes of protein kinase gene expression in cerebral ischemia and reperfusion rats and the effect of acupuncture intervention based on Cx43 semi-channel. [Methods] According to the random number table Wistar rats were randomly divided into normal group, model group, the non-acupuncture group and the acupuncture group. Using a modified intraluminal left middle cerebral artery in rat focal cerebral ischemia-reperfusion model, the model group and the two acupuncture groups divided into four subgroups: 30 min ischemia-reperfusion group, ischemia-reperfusion 60 min group, ischemia-reperfusion group 180 min and 360 min ischemia-reperfusion groups. Electrical stimulation of acupuncture group were "top middle" and "top next line"; non-acupoint group were ipsilateral iliac crest on the ribs 10 mm fixed point as acupuncture points; model group did not receive any treatment. Quantitative PCR technology was applied to measure the expression of PKC, CK1and PP2B in the hippocampus. [Results] Compared with the normal group and sham operation group, the expression of PKC mRNA, CK1 mRNA and PP2B mRNA in model group showing a rising trend; compared with the model group at the same time point, and there was no significant change of PKC mRNA expression in the non-acupuncture group (P>0.05). Tiaoshen Tongluo acupuncture can significantly reduce its expression at each time point, compared with the model group and the non-acupuncture group, and the difference was statistically significant (P<0.05). [Conclusion] The Tiaoshen Tongluo acupuncture method may inhibit the expression of PKC mRNA, CK1 mRNA and PP2B mRNA in cerebral ischemia reperfusion rat. Through regulating of key factor phosphorylation of Cx43 phosphate, to control the opening of the semi-channel, playing a neuroprotective role in ischemic brain injury. |
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