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| Inhibitory effect of Baicalin on influenza virus A H1N1 in vitro |
| Hits 1720 Download times 1696 Received:November 20, 2014 |
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| DOI
10.11656/j.issn.1672-1519.2015.04.10 |
| Key Words
Baicalin;antiviral;in vitro;A549;H1N1 |
| Author Name | Affiliation | E-mail | | LIU Xiao-ting | Laboratory of Chinese Medicine on Viral Disease, Beijing University of Chinese Medicine, Beijing 100029, China | | | ZHANG Yi | Laboratory of Chinese Medicine on Viral Disease, Beijing University of Chinese Medicine, Beijing 100029, China | | | GU Li-gang | Laboratory of Chinese Medicine on Viral Disease, Beijing University of Chinese Medicine, Beijing 100029, China | lggulg@163.com | | WU Jun | Laboratory of Chinese Medicine on Viral Disease, Beijing University of Chinese Medicine, Beijing 100029, China | | | QIU Ze-ji | Laboratory of Chinese Medicine on Viral Disease, Beijing University of Chinese Medicine, Beijing 100029, China | | | WANG Yue-qi | School of Pre-clinical Medicine, Beijing University of Chinese Medicine, Beijing 100029, China | |
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| Abstract
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| [Objective] To investigate the inhibitive effect of Baicalin on influenza A H1N1 virus infection in epithelial cell line A549. [Methods] The morphologic study and cytopathogenic effect (CPE) of cells were observed through inverted microscope. The maximum non-toxic concentration (TC0) and the median toxic concentration (TC50) were detected were assayed by MTT method. Furthermore, to identify the best method of administration and action time of baicalin, A549 cell infected with H1N1 were detected by using MTT method. The median inhibitory concentration (IC50) and treatment indexes (TI) of the best method of baicalin on viral infection were calculated with Probit regression. [Results] Baicalin had a significant inhibitory effect on influenza A H1N1 virus infection in epithelial cell line A549. The survival rate of A549 was significantly higher which added baicalin from 7.920 to 0.495 μg/mL compared with the model without the drug. In addition, the best method of baicalin against viral infection were added 2 hours after cells infected and treated 48 hours. The best survival rate was 89.107% with 3.960 μg/mL of baicalin. In this administrated method, IC50 was 1.399 μg/mL, and TI was 22.608. [Conclusion] Patients suffered from CAP with chest CT consolidation shadows can be benefited from the use of the safflower yellow injection and the safflower yellow injection can be helped for the absorption of chest CT-suggested consolidation shadows. |
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