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| The effects of psoralen components on mice osteoblast and osteoclast differention in vitro |
| Hits 1569 Download times 1920 Received:November 29, 2014 |
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| DOI
10.11656/j.issn.1672-1519.2015.05.12 |
| Key Words
isopsoralen;isobavachalcone;osteoclasts;osteoblast;proliferation;differention |
| Author Name | Affiliation | | CHAI Li-juan | Tianjin Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | FAN Na | Tianjin Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | WANG Hong | Tianjin Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Tianjin Key Laboratory of Chinese Medical Pharmacology, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | ZHANG Han | Tianjin Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | WANG Shao-xia | Tianjin Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Tianjin Key Laboratory of Chinese Medical Pharmacology, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | MIAO Lin | Tianjin Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | MAO Hao-ping | Tianjin Key Laboratory of Chinese Medical Pharmacology, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | ZHOU Kun | Tianjin Key Laboratory of Chinese Medical Pharmacology, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China |
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| Abstract
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| [Objective] To study the effects of psoralen components, isopsoralen and isobavachalcone on the cultured osteoclasts TRACP enzyme activity and differention. Moreover, the effect of two components on the mice osteoblasts proliferation and differentiation were also studied. [Methods] Using M-CSF and RANKL, osteoclast precursor cells were induced to differentiated into mature osteoclasts. During the differention period, isopsoralen and isobavachalcone were added to cells, therefore PNPP were used to detected osteoclasts TRACP activity. ICC staining of TRACP were performed to identify mature osteoclasts number. The neonatal mice osetoblast cells were cultured in vitro, CCK-8 methods were adopted to detected the osteoblast proliferation properties. Sodium phenylphosphate were used to measure the osteoblast marker enzyme ALP activity. [Results] Mouse osteoclasts and osteoblasts were cultured successfully in vitro. Isobavachalcone significantly inhibit the mature osteoclast formation at the dose of 0.05 μM, and isopsoralen had a significant role in promoting osteoblast proliferation at the dose of 0.1 μM. [Conclusion] Psoralea components isopsoralen and isobavachalcone have effects on bone cells, suggesting that Fructus Psoraleae maybe act as a drug for anti-osteoporosis or anti-bone resorption. |
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