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| Preparation and characterization of TanshinoneⅠ-loaded Liposomes |
| Hits 1615 Download times 1707 Received:January 23, 2015 |
| View Full Text View/Add Comment Download reader |
| DOI
10.11656/j.issn.1672-1519.2015.05.14 |
| Key Words
TanshinoneⅠ;Liposomes;entrapment efficiency |
| Author Name | Affiliation | E-mail | | GUO Li-li | Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | FAN Li-li | Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | WANG Ai-chao | Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | PANG Xiao-chen | Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | LUO Pei | Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | YANG Hong-yun | Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Insitute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin 300193, China | | | WANG Shao-xia | Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Insitute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin 300193, China | | | LIU Zhi-dong | Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | lonerliuzd@163.com |
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| Abstract
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| [Objective] To prepare TanshinoneⅠ-loaded Liposomes (TSⅠ-LP) and investigate its entrapment efficiency (EE), particle size, Zeta potential and other physicochemical properties. [Methods] TSⅠ-LP were prepared by film dispersion method, and using Sepharose gel and UV spectrophotometry to determine the entrapment efficiency of TSⅠ-LP. Differential scanning calorimetry (DSC) thermograms of the liposomal samples were recorded respectively. [Results] A good linear relationship was showed of TSⅠin the range of 1.004~6.024 μg/mL (r=0.999 9). Sepharose gel was well in separating the liposomes from free TSⅠ. The average recovery rate was (98.23±0.02)%. The EE of TSⅠ-LP was (92.56±0.39)%. The particle size was (90.64±1.21) nm and the Zeta potential was (-35.37±0.84) mV. [Conclusion] The film dispersion method can be used to prepare TSⅠ-LP with a high EE, a narrow size distribution and a stationary potential. The method which determine the content of TSⅠ-LP is reliable and sensitive. |
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