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| Effects of Huatan Tongluo formulae on expression of Beclin1 and LC3 on gene neuronal autophagy pathway in rat cortex accepted thrombolysis therapy after acute cerebral infarction |
| Hits 1444 Download times 1910 Received:December 28, 2014 |
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| DOI
10.11656/j.issn.1672-1519.2015.06.09 |
| Key Words
Huatan Tongluo formulae;rt-PA;thrombolysis;acute cerebral infarction;autophagy pathway;Beclin1;LC3 |
| Author Name | Affiliation | E-mail | | ZHANG Lin-lin | The Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300150, China | | | ZHOU Zhen | The Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300150, China | zhouzhen7681@126.com | | ZHANG Yu-lian | The Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300150, China | | | WANG Xue-yan | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | WANG Kun | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | WANG Kai | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | |
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| Abstract
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| [Objective] To observe the effects of Huatan Tongluo formulae on expression of Beclin1 and LC3 on gene neuronal autophagy pathway in rat cortex accepted thrombolysis therapy after the acute cerebral infarction. [Methods] The 160 healthy male SD rats were randomly divided into sham group, model group, rt-PA group, rt-PA-Huatan Tongluo group( Chinese medicine group for short), each group was divided into four time phases: 6 hours, 1 day, 3 days and 7 days. In the corresponding time points prepares the MCAO rat model, the rats in rt-PA group were injected rt-PA(concentration of 5.67 g/kg), and those of Chinese medicine group were given rt-PA and Chinese medicine Huatan Tongluo, 2 times a day. Then respectively observe the protein expression of Beclin1 and LC3 by Western Blot. [Results] The results of Western Blot showed: compared with sham group, the expression of Beclin1 and LC3 in model group increased significantly(P<0.05), compared with model group in the same time phases, the expression of Beclin1 and LC3 decreased in rt-PA group and Chinese medicine group(P<0.05),especially in Chinese medicine group. [Conclusion] Huatan Tongluo formulae can inhibit the activation of Beclin1 and LC3 on gene neuronal autophagy pathway, and inhibit the nerve cells excessive autophagy at a certain extent, to better prevention and treatment of reperfusion injury after acute cerebra accepted thrombolysis. |
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