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| Study of the mechanism of Fufang Xueshuantong capsule on kidney function |
| Hits 1437 Download times 1399 Received:December 25, 2014 |
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| DOI
10.11656/j.issn.1672-1519.2015.06.11 |
| Key Words
Fufang Xueshuantong capsule;gene array;diabetic nephrapathy;podocyte |
| Author Name | Affiliation | E-mail | | ZHANG Qian | Department of Endocrinology, Peking Union Medical College Hospital, Key Laboratory of Endocrinology, Ministry of Health, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, China | | | XIAO Xin-hua | Department of Endocrinology, Peking Union Medical College Hospital, Key Laboratory of Endocrinology, Ministry of Health, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, China | xiaoxinhua@medmail.com.cn | | LI Ming | Department of Endocrinology, Peking Union Medical College Hospital, Key Laboratory of Endocrinology, Ministry of Health, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, China | | | LI Wen-hui | Department of Endocrinology, Peking Union Medical College Hospital, Key Laboratory of Endocrinology, Ministry of Health, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, China | | | YU Miao | Department of Endocrinology, Peking Union Medical College Hospital, Key Laboratory of Endocrinology, Ministry of Health, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, China | | | ZHANG Hua-bing | Department of Endocrinology, Peking Union Medical College Hospital, Key Laboratory of Endocrinology, Ministry of Health, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, China | | | PING Fan | Department of Endocrinology, Peking Union Medical College Hospital, Key Laboratory of Endocrinology, Ministry of Health, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, China | | | YANG Guo-hua | Department of Endocrinology, Peking Union Medical College Hospital, Key Laboratory of Endocrinology, Ministry of Health, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, China | |
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| Abstract
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| [Objective] To explore the effects of Fufang Xueshuantong capsule(FXST) on the kidney function in diabetic nephropathy (DN) rats and to investigate its possible mechanism. [Methods] SD rats were fed with high fat diet and injected with STZ to make diabetic nephropathy(DN) model. DN rats were randomly divided into four groups: low dose (XSTL), medium dose(XSTM), high dose(XSTH) FXST groups(treated with 0.3, 0.6, 1.2 g/kg/d FXST, respectively, n=10), and DN model group(treated with saline, n=10). Normal rats were enrolled as normal group(treated with saline, n=10). The fasting blood glucose, weight, 24-h urinary albumin, urinary creatinine, blood creatinine and blood urea nitrogen were tested. Illumina Rat Ref-12 Expression BeadChip gene array experiment was done using kidney of rats. [Results] FXST could reduce 24-h urinary albumin, serum creatinine and serum urea nitrogen, and increase urinary creatinine in a dose dependent manner compared with those of DN model group(P<0.05 or P<0.01). Gene array showed that FXST significantly changed expression of genes(34 increased, 33 decreased). Real-time PCR showed that relative levels for Col1a1, Ctgf and Tgfb1 were significantly decreased in FXST group, compared with the model group. Cyp2c23 and Nphs1 were increased. [Conclusion] FXST can moderate the kidney function in DN rats. The mechanism may be involved with BMP2-TGFβ-CTGF pathway, CYP2C23 and podocytes proteins. |
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