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| The activation of SUMO-2/3 mediated protection effects of Salvia miltiorrhiza on ischemic neurons |
| Hits 1587 Download times 1449 Received:January 28, 2015 |
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| DOI
10.11656/j.issn.1672-1519.2015.07.10 |
| Key Words
ischemic cerebrovascular disease;small ubiquitin like protein;Salvia miltiorrhiza;neuron |
| Author Name | Affiliation | E-mail | | WANG Hui-gang | Xiaowangzhuang Community Health Service Center, Dagang District, Binhai New Area of Tianjin, Integrated Traditional Chinese and Western Medicine, Tianjin 300273, China | | | YU Si-miao | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | QIU Jin | Neurosurgenry Department, Tianjin Anjie Linao Brain Hospital, Tianjin 300402, China | | | LIU Zhen-lin | Neurosurgenry Department, Tianjin Anjie Linao Brain Hospital, Tianjin 300402, China | wjzhenlin817@163.com |
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| Abstract
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| [Objective] To study the protection mechanism of salvia miltiorrhiza on neurons after ischemic cerebral vascular disease from the field of small ubiquitin-like protein (SUMOs). [Methods] The neurons were cultured from the hippocampal of SD rats, and identified by fluorescence immunoassay method. After making oxygen glucose deprivation (OGD) model for neurons, we designed three groups for this study: control group, OGD group and salvia miltiorrhiza group. The lactate dehydrogenase (LDH) content was detected by ELISA, and apoptosis by apoptosis in situ. Then the quantitative expression and intracellular localization of SUMO-1and SUMO-2/3 proteins were detected by western blot and immunofluorescence method. [Results] The cultured cells were with high expression of neuron specific enolase (NSE) and microtubule associated protein-2 (MAP-2), but low level of glial fibrillary acidic protein (GFAP), the neuron purity greater than 95%. ELISA results showed that the content of LDH in the three groups were control group < Salvia miltiorrhiza group P<0.05), but there was no significant change in SUMO-1 (P>0.05), and salvia miltiorrhiza treatment can further improve the expression of SUMO-2/3 (P<0.05). Immunohistochemistry results showed that there was apparent shift of SUMO-2/3 from cytoplasm to nucleus in OGD neurons, and salvia miltiorrhiza treatment could further enhance this effect. [Conclusion] Salvia miltiorrhiza can induce SUMO-2/3 activation and enhance protect effects on ischemic neurons. |
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