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| Effects of acupuncturing on spontaneously hypertensive rats blood pressure and antihypertensive mechanism |
| Hits 1526 Download times 1497 Received:February 25, 2015 |
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| DOI
10.11656/j.issn.1672-1519.2015.08.12 |
| Key Words
hypertension;acupuncture;reactive oxygen species;nitric oxide;endothelial nitric oxide synthase |
| Author Name | Affiliation | | LIU Jian | Acupuncture Institute, The First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Acupuncture Encephalopathy Key Research Laboratory of the State Administration of Traditional Chinese medicine, Three-level Laboratory of Dose-effect relationship of the State Administration of Traditional Chinese Medicine, Tianjin Acupuncture Key Laboratory, Tianjin 300193, China | | GAO Ting | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | WAN Ji-wei | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | LI Yin-hong | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | WANG Min | Acupuncture Department, the First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China |
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| Abstract
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| [Objective] To observe the effects of acupuncture on spontaneously hypertensive rat(SHR) blood pressure, ROS and NO/eNOS, and research the antihypertensive mechanism from the perspective of resistancing to oxidative stress. [Methods] To separate the SHRs into acupuncture group and model group randomly, WKYs as normal blood pressure controlled group. To acupuncture group, acupuncture with the twirling manipulation, frequency 120～160 times/min, lasting 1 min. Model group and normal group were treated by the same crawl stimulation. The systolic pressure was measured before and after the treatment every day. The blood pressure was measured before and after the treatment every day. After 4 weeks of treatment, all the rats were decapitated and get the thoracic aorta. The content of ROS and NO was measured by ELISA method and nitrate reductive enzymatic. The eNOS protein express was measured by Western blotting. [Results] After 4 weeks acupuncture, the both systolic and diastolic blood pressure of SHR were lower than before, and lower than model. Among them, the systolic blood pressure lowering was more apparent. The content of ROS in SHR was higher than WKY, NO and eNOS was lower (P<0.05). After acupuncture, the content of ROS was lower and NO was higher(P<0.05), and eNOS protein was also rising trend compared with model group. [Conclusion] Acupuncture can reduce SHR systolic and diastolic blood pressure and its mechanisms may be related to reduce the content of ROS and increase the content of NO/eNOS, thus improve the antioxidant capacity. |
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