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| Determination of the residual organic solvents in Salvianolic acid B by NMR |
| Hits 1808 Download times 1676 Received:January 05, 2015 |
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| DOI
10.11656/j.issn.1672-1519.2015.08.15 |
| Key Words
liquid NMR;residua1 organic solvent;Salvianolic acid B;quantitative analysis |
| Author Name | Affiliation | E-mail | | XU Lei | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Research and Development Center of Traditional Chinese Medicine, Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin 300457, China | | | WANG Meng | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | WANG Yue-fei | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | JIANG Miao-miao | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | miaomiaojiang@126.com |
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| Abstract
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| [Objective] To establish an QNMR method for the determination of methanol, ethanol and acetic acid in Salvianolic acid B. [Methods] The 1H-NMR spectra was performed on a Bruker AV Ⅲ 600 MHz NMR spectrometer with a proton frequency of 600.23 MHz and a temperature of 298 K. [Results] Each experiment consisted of 16 scans with a spectral width of 12 019.2 Hz and a recycle delay of 15 s per scan. All the calibration curves showed good linearity (r2>0.999 5) in the range of 1.22~2 430 mg/L. The average recoveries were 105%~109%(n=6). [Conclusion] The method is rapid and accurate, which is helpful for the determination of the residua1 organic solvents in Salvianolic acid B. |
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