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| Preliminary study on the impact of Glycyrrhizae Radix on the growth of Trametes robiniophila mycelium and polysaccharide in liquid fermentation |
| Hits 2146 Download times 1589 Received:March 29, 2015 |
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| DOI
10.11656/j.issn.1672-1519.2015.09.14 |
| Key Words
Trametes robiniophila;liquid fermentation;polysaccharide |
| Author Name | Affiliation | E-mail | | FENG Shan | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | LI Sai-nan | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | JIANG Lin-qian | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | CHANG Yan-xu | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | MA Lin | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | malin7983@163.com |
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| Abstract
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| [Objective] To explore the best addition amount of Glycyrrhizae Radix and fermentation time of the liquid fermentation of Trametes robiniophila-Glycyrrhizae Radix, with mycelial biomass and fungi polysaccharide as the main indicators. [Methods] Mycelial of Trametes robiniophila was obtained in liquid fermentation, with Glycyrrhizae Radix added to liquid fermentation medium. The content of polysaccharides was determined by phenol-sulfuric acid method and DNS method. [Results] The best addition amount of Glycyrrhizae Radix was 8 g/L and the best fermentation time was 192 h when the biomass and polysaccharide of Trametes robiniophila got to the maximum. [Conclusion] Using Trametes robiniophila-Glycyrrhizae Radix liquid fermentation could increase the mycelial biomass and the content of intracellular and extracellular polysaccharides of Trametes robiniophila. It provides a theoretical basis for improving the yield of liquid fermentation. |
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