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| Effects of Buyang Huanwu decoction on the expression of MMP-2/9, TIMP-1, TNF-α in hypoxia induced cardiac fibroblasts |
| Hits 1492 Download times 1497 Received:May 21, 2015 |
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| DOI
10.11656/j.issn.1672-1519.2015.10.07 |
| Key Words
Buyang Huanwu decoction;cardiac fibroblast;hypoxia;MMP-2;MMP-9;TIMP-1;TNF-α |
| Author Name | Affiliation | E-mail | | JIN Xin-yao | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | ZHU Zheng | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | CHEN Na | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | LI Na | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | PANG Xiao-li | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | WANG Yi-jing | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | JIANG Xi-juan | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | xijuanjiang@foxmai.com.cn |
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| Abstract
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| [Objective] To observe the effect of Buyang Huanwu decoction on the paracrine of cardiac fibroblasts MMP-2, MMP-9, TIMP-1 and TNF-α in mice with hypoxia and explore the molecular mechanism in protecting myocardial cells. [Methods] Culture cardiac fibroblasts of neonatal mice, divided into three groups: control group, model group, traditional Chinese medicine (TCM) group, copied the anoxic model in condition of 5% CO2 + 94% N2 + 1% O2 for 24 h. MMP-2, MMP-9, TIMP-1 and TNF-α mRNA and protein expression were detected by RT-PCR and ELISA. [Results] Compared to control group, the mRNA and protein expression of MMP-2, MMP-9 and TNF-α in model group were increased, while the TIMP-1's expression were induced. After Buyang Huanwu decoction intervention, the MMP-2, MMP-9 and TNF-α in TCM group were decreased, and the TIMP-1 was increased (P<0.05). While compared to model group TNF-α had no significant difference (P>0.05). [Conclusion] Buyang Huanwu decoction can reduce the expression of MMP-2, MMP-9 and increase TIMP-1 in hypoxic cardiac fibroblasts to protect myocardial cells, prevent and relieve the development of myofibrosis. |
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