|
| Influence of Astragalus on cell apoptosis induced by high glucose in human proximal renal tubular epithelial cells |
| Hits 1544 Download times 1336 Received:May 20, 2015 |
| View Full Text View/Add Comment Download reader |
| DOI
10.11656/j.issn.1672-1519.2015.10.08 |
| Key Words
diabetic nephropathy;renal tubular epithelial cell;Astragalus injection;cell apoptosis |
| Author Name | Affiliation | E-mail | | MEI Sha-sha | Department of Traditional Chinese Medicine, Renmin Hospital of Wuhan University, Wuhan 430060, China | | | SONG En-feng | Department of Traditional Chinese Medicine, Renmin Hospital of Wuhan University, Wuhan 430060, China | songef@126.com | | XIANG Qiong | Department of Traditional Chinese Medicine, Renmin Hospital of Wuhan University, Wuhan 430060, China | |
|
| Abstract
|
| [Objective] To investigate the influence of Astragalus on cell apoptosis induced by high glucose in human proximal renal tubular epithelial cells (HK-2). [Methods] Human renal proximal tubular cells were cultured and divided into five groups: low glucose as control group, high glucose group, Astragalus pretreatment group with concentration of 2, 20 and 200 μg/mL. Each group has three samples, cultured in 37 ℃ incubator for 24, 48 and 72 hours, and collected cells and cell supernatant fluid. Apoptosis rate was measured by flow cytometry. [Results] When cells were cultured 24, 48 and 72 h, and the apoptosis rate of control group were significantly lower than high glucose group (P<0.05). When cells were cultured 24 h, the apoptosis rate of 200 and 20 μg/mL group significantly lower than high glucose group (P<0.05). When cells were cultured 48 and 72 h, the apoptosis rate of 200, 20 and 2 μg/mL group significantly lower than high glucose group (P<0.05). The apoptosis were significant different between Astragalus injection groups (P<0.05), and the effect of Astragalus were showed in dose-dependent manner. [Conclusion] Astragalus can suppress cell apoptosis induced by high glucose in human proximal renal tubular epithelial cells, which could be a novel approach of treating diabetic nephropathy. |
|
|
|
|
|
|
|
