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| Study on HPLC/PDA/ELSD fingerprint of Rhizoma Anemarrhenae |
| Hits 1622 Download times 1350 Received:April 27, 2015 |
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| DOI
10.11656/j.issn.1672-1519.2015.10.10 |
| Key Words
Rhizoma Anemarrhenae;fingerprint;HPLC/PDA/ELSD |
| Author Name | Affiliation | E-mail | | WEI Jing-na | Tianjin High-Standard Quality Testing Lab, Tianjin 300381, China | | | LIU Zheng-hui | Tianjin High-Standard Quality Testing Lab, Tianjin 300381, China | | | ZHAO Lin-lin | Tianjin High-Standard Quality Testing Lab, Tianjin 300381, China | | | GUO Yong-ze | Tianjin Agricultural Quality Standards and Testing Technology Research Institute, Tianjin 300381, China | | | CHENG Yi | Tianjin Agricultural Quality Standards and Testing Technology Research Institute, Tianjin 300381, China | cychengyi@yahoo.com | | WEN Chun-xiu | Institute of Cash Crops, Hebei Academy of Agriculture and Forestry Science, Shijiazhuang 050051, China | |
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| Abstract
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| [Objective] To establish the HPLC/PDA/ELSD fingerprint of Rhizoma Anemarrhenae. [Methods] This experiment had been done on Waters Symmetry ShieldTM RP C18 (5 μm,4.6 mm×250 mm) analytical column of 30 ℃, with a gradient eluted acetonitrile-0.1% acetic acid mobile phase system of 1.0 mL/min. The detection wavelength had been set at 258 nm, N2 pressure was 30 Psi, and the temperature of the drift tube was 80 ℃. [Results] The mutual modes of the HPLC/PDA/ELSD fingerprint were set up under the established condition. The 17 mutual peaks in the HPLC-PDA fingerprint and 14 mutual peaks in the HPLC-ELSD fingerprint of 11 samples were confirmed. [Conclusion] The method that had been established in this experiment is a good method to totally control the quality of Rhizoma Anemarrhenae. |
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