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| Effects of “Huoxue Kaiqiao” decoction on the behavior and blood-brain barrier in middle cerebral artery occlusion rats |
| Hits 1418 Download times 1477 Received:May 29, 2015 |
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| DOI
10.11656/j.issn.1672-1519.2015.11.11 |
| Key Words
“Huoxue Kaiqiao” decoction;MCAO;blood-brain barrier;Claudin-5 protein |
| Author Name | Affiliation | E-mail | | ZHAO Jin-sheng | Department of Neurology, Tianjin Nankai Hospital, Tianjin 300100, China
Tianjin Medical University, Tianjin 300070, China | | | MENG Fan-zheng | Department of Neurology, Tianjin Nankai Hospital, Tianjin 300100, China | | | YIN Yun | Tianjin Medical University, Tianjin 300070, China | | | LI Ping | Tianjin Third Central Hospital, Tianjin 300170, China | lipingtjutcm@sina.com |
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| Abstract
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| [Objective] To investigate the time-effect relationship of “Huoxue Kaiqiao” decoction on the behavior and blood-brain barrier (BBB) in middle cerebral artery occlusion rats. [Methods] The 48 MCAO Wistar model rats were randomly divided into model group and “Huoxue Kaiqiao” decoction groups (group I: administration 2 hours after modeling;group II: administration 24 hours after modeling and group III: administration 48 hours after modeling). Each group contained 12 rats, and another 12 rats made up sham operation group. Zealonga scoring method was used to observe the neurological deficit situation of rats. Two hours before death from intravenous injection of EB solution in order to observe changes in BBB permeability, and another taking fixed brain to detect Claudin-5 protein content by immunohistochemistry method. [Results] Compared with the control group, nerve function were improved in all treatment groups (P<0.05). Cerebral ischemic striatum EB in treatment group Ⅱ and Ⅲ were lower than the model group (P<0.05), but the content of Claudin-5 in these groups were high (P<0.05). However, the difference was not statistically significant between groupⅡ and group Ⅲ, and between group I and model group (P>0.05). [Conclusion] Resuscitation promoting blood circulation by reducing BBB permeability soup can reduce damage Claudin-5 protein in rat brain barrier to protect MCAO, but the protective effect in the acute phase of the BBB is not obvious, and yet could not prove that in the non-acute phase sooner promoting blood circulation giving resuscitation BBB decoction of the more remarkable. |
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