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| Characterization of metabolites of liquirtigenin in rats by UPLC-Q-TOF/MS |
| Hits 1605 Download times 1305 Received:July 03, 2015 |
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| DOI
10.11656/j.issn.1672-1519.2015.12.14 |
| Key Words
liquirtigenin;metabolite;UPLC-Q-TOF/MS;isomerization;glucuronidation;sulfation |
| Author Name | Affiliation | E-mail | | LI Yuan-yuan | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Tianjin International Joint Academy of Biotechnology and Medicine, Research and Development Center of Traditional Chinese Medicine, Tianjin 300457, China | | | JIANG Zhen-zuo | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Tianjin International Joint Academy of Biotechnology and Medicine, Research and Development Center of Traditional Chinese Medicine, Tianjin 300457, China | | | ZHANG Lei | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Tianjin International Joint Academy of Biotechnology and Medicine, Research and Development Center of Traditional Chinese Medicine, Tianjin 300457, China | | | CHAI Xin | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Tianjin International Joint Academy of Biotechnology and Medicine, Research and Development Center of Traditional Chinese Medicine, Tianjin 300457, China | | | WANG Yue-fei | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Tianjin International Joint Academy of Biotechnology and Medicine, Research and Development Center of Traditional Chinese Medicine, Tianjin 300457, China | wangyuefei_2006@hotmail. com |
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| Abstract
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| [Objective] To systemically study metabolites of liquirtigenin in rats, an UPLC-Q-TOF/MS method was developed. [Methods] UPLC-Q-TOF/MS method was employed to identify metabolites of liquirtigenin in plasma, urine, feces and bile samples from rats after iv and ig administration of liquirtigenin (20 mg/kg). [Results] A total of 15 metabolites were characterized in those biological samples, including isoliquirtigenin, glucuronides and sulfates of (iso)liquirtigenin, davidigenin and its sulfates, methylated isoliquirtigenin, dehydrogenated liquirtigenin. The main metabolic pathways of liquirtigenin were isomerization, glucuronidation and sulfation, methylation, dehydrogenation. [Conclusion] The UPLC-Q-TOF/MS method established in this study is successfully applied to identify metabolites of liquirtigenin in rats. |
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