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| Establishment and evaluation experimental study of acute myocardial infarction in rats |
| Hits 1877 Download times 2426 Received:October 17, 2015 |
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| DOI
10.11656/j.issn.1672-1519.2016.02.08 |
| Key Words
acute myocardial infarction;animal model;echocardiography;hemodynamic |
| Author Name | Affiliation | E-mail | | HU Zhen | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | CHEN Jing-rui | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | WEI Jing | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | LI Lan | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | WANG Ling-yan | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | FAN Guan-wei | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | fgw1005@hotmail.com |
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| Abstract
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| [Objective] To explore the establishment of a stable and reliable, easy operation and high survival rates of rats with acute myocardial infarction model preparation and effective evaluation methods. [Methods] The 50 male SD rats were anesthetized, thoracotomy left anterior descending coronary artery ligation. After 24 h, observed with echocardiograph changes in left ventricular function and left ventricular catheterization rat blood dynamics. Remove the heart, TTC sections were stained; automatic biochemical analyzer to detect blood serum creatine kinase indicators. [Results] This method of surgery survival rates as high as 91.67%. After 24 h, remove the cardiac left ventricle infarcted ventricular wall visible color of light than the surrounding myocardium. In the model group, echocardiographic and hemodynamic showed left ventricular function and wall motion was obviously weaker than the sham group. TTC staining showed significant myocardial infarction in the model group. [Conclusion] This method improves the survival rate of rats with acute myocardial infarction model, the model is stable, reproducible, and can meet once ligation or ischemia-reperfusion needs. |
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