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| Effects and mechanismof geniposide pretreatment on H9C2 cardiomyocytes during hypoxia/reoxygenation injury |
| Hits 1997 Download times 1477 Received:October 21, 2015 |
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| DOI
10.11656/j.issn.1672-1519.2016.02.13 |
| Key Words
geniposide;cardiomyocyte;hypoxia/reoxygenation injury |
| Author Name | Affiliation | E-mail | | CAI Zhi-hui | Department of Basic Medicine, Luohe Medical College, Luohe 462002, China | | | YIN Li | Department of Basic Medicine, Luohe Medical College, Luohe 462002, China | | | CHANG Quan-zhong | Department of Basic Medicine, Luohe Medical College, Luohe 462002, China | cqzchang@tom.com |
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| Abstract
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| [Objective] To explore the effects and mechanism of geniposide pretreatment on H9C2 cardiomyocytes during hypoxia/reoxygenation injury. [Methods] H9C2 cardiomyocytes were cultivated in vitro and the model of hypoxia/reoxygenation injury was established. The cells were randomly divided into 3 groups:the control group, hypoxia/reoxygenation injury group and the geniposide pretreatment group. The contents of CK, LDH, MDA and the activity of SOD were evaluated by spectrophotometric method. The cell viability was measured with MTT. The apoptosis rate was detected by Annexin V-FITC/PI staining. [Results] Compared with the hypoxia/reoxygenation injury group, the geniposide pretreatment group can reduce the concentration of CK, LDH and MDA, improve the activity of SOD in myocardial tissue, improve the cell viability and reduce the apoptosis rate. [Conclusion] The geniposide has protective effects on hypoxia/reoxygenation injury, which are associated with scavenging free radical, enhancing the capacity of antioxidation and inhibiting cadioocyte apoptosis. |
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