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| Study of Xiegan Anshen method on monoamine neurotransmitters with rats after sleep deprivation |
| Hits 1888 Download times 1805 Received:November 26, 2015 |
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| DOI
10.11656/j.issn.1672-1519.2016.03.08 |
| Key Words
Xiegan Anshen method;water platform;sleep deprivation rat;monoamine neurotransmitter |
| Author Name | Affiliation | E-mail | | XING Jia | No.2 Department of Eneephalopathy, Dongfang Hospital, Beijing University of Chinese Medicine, Beijin 100078, China | | | HE Li-juan | No.2 Department of Eneephalopathy, Dongfang Hospital, Beijing University of Chinese Medicine, Beijin 100078, China | | | WANG Jia-lin | No.2 Department of Eneephalopathy, Dongfang Hospital, Beijing University of Chinese Medicine, Beijin 100078, China | | | WANG Chun-ye | No.2 Department of Eneephalopathy, Dongfang Hospital, Beijing University of Chinese Medicine, Beijin 100078, China | | | GUO Mao-juan | No.2 Department of Eneephalopathy, Dongfang Hospital, Beijing University of Chinese Medicine, Beijin 100078, China | dfguorongjuan@163.com |
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| Abstract
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| [Objective] To investigate the Xiegan Anshen intervention on monoamine neurotransmitters in brainstem and hippocampus of rats after sleep deprivation. [Methods] Selection method of water platform to cause sleep deprivation model, treatment by Xiegan Anshen decoction for 7 days, then the rats were sacrificed to get the brain, termination content of monoamine neurotransmitters in brainstem and hippocampus by High performance liquid chromatography. [Results] After sleep deprived, in brainstem, the content of 5-HIAA, 5-HT decreased significantly, content of DA, NE, HVA in brainstem increased obviously, and there was no significant change in DOPAC. The changes in the content of monoamine neurotransmitter in the hippocampus were not significant. After treatment by Xiegan Anshen decoction, the content of 5-HT, 5-HIAA in brainstem were significantly increased, the content of DA in brainstem decreased significantly and the the content 5-HT, 5-HIAA, DA, HVA in hippocampus increased, the content of NE in hippocampus decreased. After treatment by Diazepam tablets, the change of monoamine neurotransmitter in hippocampus was the same with the traditional Chinese medicine group results. But in the brainstem, the content of 5-HIAA increased, and the contents of 5-HT, DA had no obvious change. [Conclusion] Xiegan Anshen decoction can regulate the content of monoamine neurotransmitters of sleep deprivation rats, and the effects of target more than Diazepam tablets, the mechanism may be comprehensive regulating monoamine neurotransmitters, correcting the disorder. |
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