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| Protective effects and mechanisms of sofren injection on primary cardiomyocytes hypoxia-reoxygenation injury |
| Hits 1837 Download times 1895 Received:August 13, 2015 |
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| DOI
10.11656/j.issn.1672-1519.2016.03.09 |
| Key Words
sofren injection;hypoxia-reoxygenation;primary cardiomyocyte;oxidative stress |
| Author Name | Affiliation | E-mail | | WANG Ya-dong | Tianjin State Key Laboratory of Modern Chinese Medicine-Province and Ministry Co-established State Key Laboratory Cultivation Base, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | Patrick Asare Fordjour | Tianjin State Key Laboratory of Modern Chinese Medicine-Province and Ministry Co-established State Key Laboratory Cultivation Base, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | LI Lan | Tianjin State Key Laboratory of Modern Chinese Medicine-Province and Ministry Co-established State Key Laboratory Cultivation Base, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | WEI Jing | Tianjin State Key Laboratory of Modern Chinese Medicine-Province and Ministry Co-established State Key Laboratory Cultivation Base, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | FAN Guan-wei | Tianjin State Key Laboratory of Modern Chinese Medicine-Province and Ministry Co-established State Key Laboratory Cultivation Base, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | fgw1005@hotmail.com |
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| Abstract
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| [Objective] To investigate the effect of sofren injection on primary cardiomyocytes damaged by hypoxia-reoxygenation and to research signal pathways involved in the mechanism of sofren injection. [Methods] The cell viability was measured by MTT. Primarily cultured cardiomyocytes were pretreated with different concentrations of sofren injection, cultured in hypoxia for 9 h and reoxygenation for 2 h. The cell viability was measured by MTT. The ROS generation and mitochondrial membrane potential in the cell were measured by CMH2DCF-DA and JC-1 fluorescent probe, the vitality and gene expression of superoxide dismutase (SOD). [Results] Compared with hypoxia-reoxygenation group, pretreated with different concentrations of sofren injection significantly attenuated model-induced cell viability and mitochondrial membrane potential loss, dramatically decreased the ROS generation. The SOD vitality and gene expression were increased by sofren injection. [Conclusion] Sofren injection can protect rat cardiomyocytes from the injury induced by hypoxia-reoxygenation. |
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