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| Curcumin extract influence on tumor angiogenesis of A549 cell subsets SP and NON-SP cells |
| Hits 1878 Download times 1419 Received:September 25, 2015 |
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| DOI
10.11656/j.issn.1672-1519.2016.03.10 |
| Key Words
curcumin;VEGF;NF-κB;human lung adenocarcinoma A549;NON-SP cell;SP cell |
| Author Name | Affiliation | | LI Xiao-jiang | The First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | JIA Ying-jie | The First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | ZHANG Wen-zhi | Tianjin Neurosurgery Institute, Tianjin 300060, China | | ZHANG Ying | The First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | XU Wen-ting | Graduate School, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China |
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| Abstract
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| [Objective] Through the animal experiment further understand the main ingredient in turmeric extract curcumin-of tumor angiogenesis of the specific mechanism. [Methods] The 40 BALB/C mice were divided into four groups of male nude, one group is only 10. group A (SP and group of cells curcumin group), group B (SP and group of cells tumor-burdened control group), group C (as SP and group of cells curcumin group), group D (as SP and group of cells tumor-burdened control group). A and B two groups in lung adenocarcinoma before the establishment A549SP cells subsets tumor-burdened model, C, D two groups establish lung adenocarcinoma A549 cells subsets as SP tumor-burdened model, and after 16 days the model are observated, in group A, C intraperitoneal injection of mice curcumin, the next day 1, eight times, B and D two groups of injection saline. 16 days later executed mice, will tumor piece of tissue dissection, comparison of the heavy; immunohistochemical method to detect tumor tissue VEGF, CD34, NF-kB expression; PT-PCR detected Notch1 mRNA content. [Results] The group of cells SP curcumin group of suppression effect was better than the group of cells as SP curcumin group that cancer stem cells and tumor angiogenesis closely related. [Conclusion] Curcumin can inhibit tumor growth, and may inhibit the expression of NF-kB, down regulate the content of mRNA Notch, block the Notch1 signaling pathway and inhibit the expression of VEGF in tumor tissues. |
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