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| Study on the anti-influenza virus H1N1 activity of microemulsion extract of cmpound Longqincao in vitro |
| Hits 1702 Download times 1539 Received:November 03, 2015 |
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| DOI
10.11656/j.issn.1672-1519.2016.03.12 |
| Key Words
microemulsion;compound Longqincao;microemulsion extract;H1N1;influenza virus |
| Author Name | Affiliation | E-mail | | LIU Xiao-yan | School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China | | | LIU Shuai | School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China | | | LI Yan | School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China | | | GU Li-gang | Educational Ministry Key Laboratory of Preventing and Treating Viral Diseases with Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China | | | LIU Xiao-ting | Educational Ministry Key Laboratory of Preventing and Treating Viral Diseases with Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China | | | DU Hong | School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China | duhong@vip.163.com |
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| Abstract
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| [Objective] To study the antiviral effects of biocompatible microemulsion and microemulsion extract of cmpound Longqincao on H1N1 influenza virus in vitro. [Methods] The maximum non-toxic concentration (TC0) and median toxic concentration (TC50) of biocompatible microemulsion and microemulsion extract of cmpound Longqincao on A549 cells were detected by using methyl thiazolyl tetrazolium (MTT). The influences of these drugs on virus-infected cells were observed by cytopathic effect (CPE), the cytopathic inhibition rate (ER) was detected by using MTT. The median inhibitory concentration (IC50) and therapeutic index (TI) of these two drugs on viral infection were calculated by the Probit regression. [Results] Microemulsion had inhibitory effect on the pathogenic effect of influenza virus, IC50 was 769.178 mg/L (Calculated with microemulsion) and TI was 9.078. When the concentration of microemulsion extract of cmpound Longqincao was 1 250 mg/L, The antiviral rate was 91.37%. IC50 was 314.808 mg/L (Calculated with microemulsion) and TI was 20.889. It had a significant effect on protecting A549 cells from intrusion. [Conclusion] The microemulsion extract of cmpound Longqincao played a good role in anti-H1N1 influenza virus, the microemulsion also had antiviral effect, and maybe played a supporting role to a certain extent. |
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