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| The mechanism study of Yishen Huazhuo decoction on improving the learning and memory ability of rats with Alzheimer's disease by Neurogenin1 |
| Hits 2099 Download times 1822 Received:December 20, 2015 |
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| DOI
10.11656/j.issn.1672-1519.2016.04.13 |
| Key Words
Alzheimer's disease;Yishen Huazhuo decoction;ngn1;GFAP;Tubulin |
| Author Name | Affiliation | | ZHANG Lin-lin | The Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300150, China | | WANG Kai | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | SUN Wei-ming | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | WANG Yi-fu | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China |
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| Abstract
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| [Objective] To explore the influence of Yishen Huazhuo decoction (YHD) on the expression of Neurogenin1(ngn1), GFAP, and Tubulin in hippocampus of Alzheimer's disease (AD) model rats, and try to explore the mechanism of YHD in improving learning and memory of AD rats. [Methods] SD rats were randomized into five groups:sham operation group, model group, low-dosage, medium-dosage, and high-dosage YHD group. The dosage of low, medium, high YHD is 2.8, 5.6, 11.2 g/kg, respectively, once a day, four weeks in total. RT-PCR for ngn1, western blot for GFAP and Tubulin. [Results] Compared with sham operation group, model group rats hippocampus ngn1 gene expression decreased, increased expression of GFAP protein, Tubulin protein expression were significantly decreased, with significant difference(P<0.05). Compared with model group, three YHD group hippocampus ngn1 gene expression increased, decreased expression of GFAP protein, and increased Tubulin, with significant difference. [Conclusion] YHD might increase ngn1 in hippocampus of Alzheimer's AD model rats, promote neuronal regeneration thereby improving learning and memory function. |
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