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| Experimental study on Jianpi Yifei Huatan decoction improvement airway mucus hypersecretion in COPD rats through inhibition of TNF-α signaling pathway |
| Hits 1451 Download times 1529 Received:December 22, 2015 |
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| DOI
10.11656/j.issn.1672-1519.2016.05.10 |
| Key Words
chronic obstructive pulmonary disease;mucus hypersecretion;Jianpi Yifei Huatan decoction;TNF-α;EGFR |
| Author Name | Affiliation | E-mail | | YANG Xiao-min | School of Preclinical Medicine, Beijing University of Chinese Medicine, Beijing 100029, China | | | ZHAO Na-mei | School of Preclinical Medicine, Beijing University of Chinese Medicine, Beijing 100029, China | | | LIU Juan | School of Preclinical Medicine, Beijing University of Chinese Medicine, Beijing 100029, China | | | DENG Xiu-lan | School of Preclinical Medicine, Beijing University of Chinese Medicine, Beijing 100029, China | | | WANG Qing-qing | School of Preclinical Medicine, Beijing University of Chinese Medicine, Beijing 100029, China | | | ZHANG Xiao-jing | School of Preclinical Medicine, Beijing University of Chinese Medicine, Beijing 100029, China | | | PAN Fei | School of Preclinical Medicine, Beijing University of Chinese Medicine, Beijing 100029, China | | | LI Pei | School of Preclinical Medicine, Beijing University of Chinese Medicine, Beijing 100029, China | | | ZHONG Xiang-gen | School of Preclinical Medicine, Beijing University of Chinese Medicine, Beijing 100029, China | zhongxg@bucm.edu.cn |
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| Abstract
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| [Objective] To explore the mechanism of improvement of mucus hypersecretion in COPD rats by Jianpi Yifei Huatan decoction. [Methods] The rat model of COPD was established by tobacco smoke inhalation,and then the rats were randomly divided into the normal group,model group,Jianpi Yifei Huatan decoction group,Huatan decoction group, Jianpi Yifei Huatan decoction group and carbocisteine group. The normal group and model group were given normal saline solution intragastrically and other groups were given relevant medicines for 28 days,respectively. TNF-α in bronchoalveolar lavage fluid was detected by ELISA, and phospho-EGFR, phospho-PI3K and phospho-AKT in lung were detected by Western Blot. [Results] Compared with the normal group, the expressions of TNF-α, phospho-EGFR, phospho-PI3K and phospho-AKT in model group rats were significantly higher (P<0.01). Compared with model group,the expressions of TNF-α, phospho-EGFR, phospho-PI3K and phospho-AKT in all treating group rats were significantly lower (P<0.01 or P<0.05) or with a downward tendency. [Conclusion] Jianpi Yifei Huatan decoction improves airway mucus hypersecretion in COPD rats through inhibition of TNF-α signaling pathway, and the role of "treating both symptoms and root causes" is better than single "treating symptoms" or "root causes". |
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