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| Study of Taohe Chengqi Tang on Toll-like receptor passage in diabetic vascular fibrosis with rats |
| Hits 1693 Download times 1508 Received:December 20, 2015 |
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| DOI
10.11656/j.issn.1672-1519.2016.05.11 |
| Key Words
macrovascular fibrosis;THCQT;Toll-like receptor-2;Toll-like receptor-4 |
| Author Name | Affiliation | | XU Yang | The First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | WANG Jun | The First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | WU Hai-kuo | The First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China |
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| Abstract
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| [Objective] Using Western blotting to detect the expression of TLR-2, TLR-4 which from the diabetes rats in lower limb femoral vessels, and observing by Taohe Chengqi Tang(THCQT) impact on related indicators, revealing intervention mechanisms of THCQT on diabetic vascular fibrosis. [Methods] The 170 male SD rats were divided into five groups, A- the normal control group (n=30), other 140 making diabetes model and succeed 123, randomly divided into 4 groups:B- model control group (n=31), C- Chinese high-dose treatment group (n=30), D-medicine dose treatment group (n=31), E- low dose treatment group (n=31). A group was no drug intervention, group B received 0.9% saline 10 mL/(kg·d) orally, group C was given THCQT 1.8 g/kg, D group was given THCQT 0.9 g/kg, E group was given THCQT 0.45 g/kg. Drug intervention first weeks, 12 weeks, 20 weeks, the rats were sacrificed according to plan, the groups were drawn, preserved specimens, Western blotting detection TLR-2 and TLR-4 expression. [Results] TLR-2 and TLR-4 has significant expression in diabetic rat femoral artery, TCD THCQT can effectively reduce the expression of TLR-2 and TLR-4. [Conclusion] Diabetic vascular disease may be associated with Toll-like receptor pathway and apoptosis, TCD THCQT can obviously improve diabetic vascular lesions, and slow down the fibrosis process, its intervention effect associated with dosage and duration. |
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