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| Effects of Shenzhujing tablets for sodium pentobarbital-induced hypnosis in mice |
| Hits 1440 Download times 1407 Received:March 20, 2016 |
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| DOI
10.11656/j.issn.1672-1519.2016.05.13 |
| Key Words
insomnia;Shenzhujing tablet;5-HT |
| Author Name | Affiliation | E-mail | | ZHAO Hong-jie | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | FAN Guan-wei | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | LUO Yi-yang | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | GAO Xiao-yu | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | QI Ji | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | LIU Zhan-biao | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | 634256747@qq.com | | GUO Li-ping | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | lpgtjn@163.com |
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| Abstract
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| [Objective] To observe the effects of Shenzhujing tablets on sodium pentobarbital-induced hypnosis. [Methods] The 96 of Kunming mice were randomly divided into 4 groups: normal group, and Shenzhujing tablet (0.5, 0.25, 0.125 g/kg) groups. The drugs were administrated orally in single doses to 12 of the mice in each group 2 h before the intraperitoneal injection of sodium pentobarbital at the dose of 30 mg/kg or 50 mg/kg, respectively. Then the mice received daily oral treatments of drugs for 7 days. Similarly, Sodium pentobarbital-induced hypnosis tests were also used 2 h after the last administration of the drugs. The sleep latency and sleep duration during pentobarbital-induced sleep were measured and the ratios of sleep mice to non-sleep mice were counted in mice after oral administration of the drugs. Moreover, the contents of the 5-hydroxytryptamine(5-HT) and 5-hydroxyindoleacetic acid(5-HIAA)in the brain stem were determined. [Results] Compared with normal group, Shenzhujing tablets significantly prolonged the pentobarbital-induced sleep duration and decrease sleep latency at dose of 0.5 g/kg(P<0.05), increase the ratios of sleep mice(P<0.01) to non-sleep mice and the contents of 5-HT in the brain stem under the condition of 30 mg/kg of sodium pentobarbital (P<0.05). [Conclusion] Shenzhujing tablets possess sedative-hypnotic activity possibly by promoting the secretion of 5-HT in the brain and might be effective agents for treatment of insomnia. |
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