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| Effect of the Tianma Gouteng Yin regulating the protein expression of Bax and Caspase-3 of myocardial tissue in spontaneously hypertensive rats |
| Hits 2174 Download times 1318 Received:February 28, 2016 |
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| DOI
10.11656/j.issn.1672-1519.2016.06.10 |
| Key Words
Tianma Gouteng Yin;reducing blood pressure;AngⅡ;ALD;Bax;Caspase-3 |
| Author Name | Affiliation | E-mail | | SHI Xiao-chi | Department of Cardiology, The Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300150, China | | | LIU Wei | Department of Cardiology, The Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300150, China | | | WANG Juan | Department of Cardiology, The Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300150, China | | | ZHAO Ying-qiang | Department of Cardiology, The Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300150, China | zhaoyingqiang1000@126.com |
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| Abstract
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| [Objective] To investigate the mechanism of Tianma Gouteng Yin (TMGTY) reducing blood pressure and the protein expression of Bax and Caspase-3 in myocardial tissue in spontaneously hypertensive rats. [Methods] Twenty 14-week-old SHR rats were randomly assigned to control group (n=6), TCM group (n=7) and Western medicine group (n=7). The rats in TCM group were given TMGTY with the dose of 20 g/(kg·d); those in the western medicine group were given ramipril [1 mg/(kg·d)]; those in control group were given the same volume of pure water. Blood pressure and heart rate were measured weekly. After 4 weeks, abdominal aortic blood and sacrifice animals. Enzyme-linked immunosorbent assay was used to analyze the content of AngⅡ and ALD in serum. Western blot was used to analyze quantitatively Bax and Caspase-3. [Results] 1) Starting from the third week after the administration, compared with the control group, TMGTY significantly reduced systolic blood pressure (P<0.05, P<0.01). Although not significant antihypertensive effect, TMGTY controlled the overall increase in systolic blood pressure, and systolic blood pressure gradually decreased. 2) TMGTY and ramipril had no significant effect on heart rate. 3) Compared with the control group, TMGTY and ramipril significantly reduced the content of AngⅡ and ALD in serum (P<0.01). 4) Compared with the control group, TMGTY and ramipril significantly reduced the protein expression of Bax and Caspase-3 in myocardial tissue (P<0.01). [Conclusion] TMGTY could effectively control the increase in systolic blood pressure in SHR. The antihypertensive mechanism of TMGTY may be reducing ALD and AngⅡ. Further, by reducing the protein expression of Bax and Caspase-3, TMGTY possibly inhibited the apoptosis of cardiomyocytes, and achieved protection to cardiomyocytes. |
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