|
| Study on the autophagy and resistance protein affected by Xiaoyan decoction in A549/DDP cells |
| Hits 2025 Download times 1481 Received:January 16, 2016 |
| View Full Text View/Add Comment Download reader |
| DOI
10.11656/j.issn.1672-1519.2016.06.11 |
| Key Words
Xiaoyan decoction;autophagy;resistance;molecular pathway |
| Author Name | Affiliation | E-mail | | YANG Pei-ying | The First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | XU Wen-ting | The First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | LIU Hong-gen | The First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | ZHANG Xin | The First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | ZHANG Ying | The First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | YU Xiao-yu | The First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | JIA Ying-jie | The First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | jiayingjie1616@sina.com |
|
| Abstract
|
| [Objective] Through the cisplatin resistance of the human lung adenocarcinoma cells A549/DDP multi-resistant and influence of autophagy protein by Xiaoyan decoction, finding out the influence of elimination of cisplatin resistance of lung adenocarcinoma A549/DDP resistance reversal agents and explore relevant molecular pathways. [Methods] Establish a stable cell resistance model, continuous lavaging Xiaoyan decoction with different concentrations in 10 days, by serum pharmacology experiment method with drug-containing serum on lung adenocarcinoma cells resistant cells A549/DDP, through the QRT-PCR and Werstern Blot western Blot method to detect A549/DDP resistant, autophagy protein expression of related genes and related pathways. [Results] Resistance effect on lung adenocarcinoma cells A549/DDP with high dose Xiaoyan decoction, low dose Xiaoyan Decoction and normal saline, elimination saline resistance effect on lung adenocarcinoma cells A549/DDP, detecting drug-resistant related proteins in A549/DDP cell P-gp, LRP and Beclin1 protein involved in autophagy, HMGB1mRNA and protein expression changes,through the detection of A549/DDP cells drug-resistant related proteins P-gp, LRP and Beclin1 protein involved in autophagy, HMGB1mRNA and protein expression changes, high dose group of rock soup elimination processing cell lines after P-gp, LRP and Beclin1 protein involved in autophagy, both the HMGB1 mRNA and protein were lower (P<0.05). Further found that Xiaoyan decoction can affect the AKT1-elimination mTOR related genes and significantly reduce the AKT1, mTOR protein expression. [Conclusion] We hypothesized that Xiaoyan decoction influence elimination drug-resistant related proteins P-gp, LRP and Beclin1 protein involved in autophagy, HMGB1 genetic changes by AKT1- mTOR molecular pathways. |
|
|
|
|
|
|
|
