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| Study on the mechanism and the effects of prokinetic agents combined with Chinese herbs on the delayed gastric emptying in diabetic rats |
| Hits 1744 Download times 1330 Received:March 28, 2016 |
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| DOI
10.11656/j.issn.1672-1519.2016.06.12 |
| Key Words
Chinese herb;myenteric nerve plexus;diabetic;gastric emptying |
| Author Name | Affiliation | | ZHANG Yi-hong | Department of Dastroenterology, Tianjin Beichen Hospital, Tianjin 300400, China | | YANG Guo-li | Department of Dastroenterology, Tianjin Beichen Hospital, Tianjin 300400, China | | FU Ying | Department of Dastroenterology, Tianjin Beichen Hospital, Tianjin 300400, China | | ZHANG Zhao | Department of Dastroenterology, Tianjin Nankai Hospital, Tianjin 300100, China | | CAO Ze-wei | Department of Dastroenterology, Tianjin Nankai Hospital, Tianjin 300100, China | | ZHANG Jun | Department of Dastroenterology, Tianjin Beichen Hospital, Tianjin 300400, China | | WANG Tian-xi | Department of Dastroenterology, Tianjin Nankai Hospital, Tianjin 300100, China | | GUO Na | Department of Dastroenterology, Tianjin Nankai Hospital, Tianjin 300100, China | | GAO Jun-qin | Department of Dastroenterology, Tianjin Beichen Hospital, Tianjin 300400, China |
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| Abstract
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| [Objective] To observe the effects of prokinetic agents combined with Chinese herbs on the delayed gastric emptying in diabetic rats and the underlying mechanism. [Methods] Totally 70 male SD rats were randomly divided into two groups: normal group (group A, n=10) and model group (n=60). The rats of model group was established as diabetes model by intraperitoneal injection of alloxan. The successful model rats were divided into four groups: control group (group B, n=15), Chinese herbs treatment group (group C, n=15), Itopride treatment group (group D, n=14), Itopride combined with Chinese herbs treatment group (group E, n=14). According to the requirements of each group, the corresponding drugs were given to the stomach every day. After 24 weeks of feeding, all rats were given 13C-gastric half-emptying time. Then all rats were killed. HE and nNOS immunohistochemical staining were used to observe the pathological changes of gastric wall. [Results] 1) Compared with the rats in group A, the gastric emptying time of all rats in model group (group B, C, D and E) were prolonged (P< 0.01). 2) Compared with group B, the gastric emptying time in rats of group C, D and E were significantly reduced (P<0.05). Compared with Group D and C, they were significantly reduced in group E (P<0.01). 3) Compared with group A, the gastric smooth muscle cell in the model group became thinner, and the number of muscle plexus and the expression of nNOS in gastric smooth muscle of rats were significantly decreased in group B, C, D and E. 4) The number of muscle plexus in group B was significantly higher than that in group D, while the number in group group D was significantly lower than that in C and group E. Meanwhile, the number of nNOS positive nerve plexus in group D was significantly lower than that in group E. [Conclusion] Combined with Chinese herbs, prokinetic agents can promote the gastric emptying in diabetic rats effectively, and the effects are better than that of single use of Chinese herbs or prokinetic agents. The mechanism may correlated with its protective effects on smooth muscle and myenteric nerve plexus. |
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