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| Simultaneous determination of coumarins and fingerprint analysis in Cnidium monnieri (L.) Cuss. |
| Hits 2426 Download times 1950 Received:January 18, 2016 |
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| DOI
10.11656/j.issn.1672-1519.2016.06.13 |
| Key Words
HPLC;Cnidium monnieri (L.) Cuss.;fingerprint;coumarins |
| Author Name | Affiliation | E-mail | | SONG Li-jiao | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | LI Jin | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | JIANG Yan | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | CHEN Ying | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | MA Lin | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | JIN Hua | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | CHANG Yan-xu | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | tcmcyx@126.com |
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| Abstract
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| [Objective] A fingerprint analysis and simultaneous determination of six coumarins were established and validated to evaluate the quality of different batches of Cnidium monnieri (L.) Cuss. [Methods] The contents of xanthotoxin, isopimpinelline, bergapten,imperatorin, osthole and isoimperatorin were determined by HPLC. The analysis was carried out on Hedra ODS-2 column(4.6 mm × 250 mm, 5 μm). The mobile phase was methanol-0.1% formic acid water aqueous solution in gradient elution. The flow rate was 0.5 mL/min and the detection wave length was 320 nm. [Results] The contents of six components in various bathes of samples were determined and HPLC fingerprint was established. The similarities of 17 batches of Cnidium monnieri (L.) Cuss. were more than 0.980. [Conclusion] The established method could be suitable to evaluate the quality control of Cnidium monnieri (L.) Cuss. |
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