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| Effect of Purendan on the pancreatic β cell secretion and IR in pancreas micro-circulatory damage in rats with T2DM |
| Hits 1866 Download times 1514 Received:March 26, 2016 |
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| DOI
10.11656/j.issn.1672-1519.2016.07.09 |
| Key Words
Purendan supermicro powder;T2DM;pancreas micro-circulatory;pancreatic β cell;insulin resistance |
| Author Name | Affiliation | | LIU Zu-han | Pharmacy Department, Peking University International Hospital, Beijing 102206, China | | ZHANG Zi-qian | Department of Drug Metabolism, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China |
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| Abstract
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| [Objective] To investigate the effect of Purendan (PRD) supermicro powder on the pancreatic β cell secretion in pancreas micro-circulatory damage in rats with T2DM, and explore the regulation mechanism for PRD in pancreatic β cell secretion.[Methods] Male wistar rats were administered intragastrically with fat milk and STZ and injected intravenously via the lateral caudal vein at a dose of 30 mg/kg. After 1 week, rats were randomly assigned to model group, PRD group, rosiglitazone maleate tablets group, and Jiangtang Tongmai tablet group. The 28 male wistar rats as control were also engaged in the experiment. Treatment were exerted according to grouping principles, and rats in control group and model group were administered intragastrically with the same dose of distilled water for 4 weeks. Then, oral glucose tolerance test, intravenous injection glucose-insulin release test, FINS-AUC/FBG-AUC, pancreatic β cell functional assessment and index of correlation were analyzed.[Results] In oral glucose tolerance test experiment, blood glucose in PRD group rats decreased within 2 h and was close to normal control, PG2 h approximated to plasma glucose level before given oral glucose[PG0 h:(14.80±1.42);PG2 h:(16.17±2.91) mmol/L]. In intravenous injection glucose-insulin release test, the significant secretion peak at the first phase were observed after 5 min in PRD treatment group, beside, secretion peak at the second phase were observed after 30 min, which conform to rapid and long pulsatile insulin secretion fashion. PRD significantly increased FINS-AUC/FBG-AUC(P<0.01). Meanwhile pancreatic β cell secretion were up-regulated, insulin secretion and sensitivity increased, and insulin resistivity decreased in PRD treatment group.[Conclusion] PRD supermicro powder possibly imporved glucose tolerance and insulin secretion in both phase via recovery of pancreatic β cell damage. |
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