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| Determination of Notoginsenoside R1, Ginsenoside Rg1, Rb1, Rd and Lobetyolin in Wenxin Keli by HPLC |
| Hits 2015 Download times 1691 Received:January 12, 2016 |
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| DOI
10.11656/j.issn.1672-1519.2016.07.13 |
| Key Words
Wenxin Keli;HPLC;Notoginsenoside R1;Ginsenoside Rg1;Lobetyolin Ginseno-side Rb1;Ginsenoside Rd |
| Author Name | Affiliation | E-mail | | NAN Guo | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Research and Development Center of Traditional Chinese Medicine, Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin 300457, China | | | ZHANG Peng | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Research and Development Center of Traditional Chinese Medicine, Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin 300457, China | | | WANG Meng | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Research and Development Center of Traditional Chinese Medicine, Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin 300457, China | | | WANG Yue-fei | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Research and Development Center of Traditional Chinese Medicine, Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin 300457, China | | | ZHU Yan | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Research and Development Center of Traditional Chinese Medicine, Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin 300457, China | | | WU Hong-hua | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Research and Development Center of Traditional Chinese Medicine, Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin 300457, China | wuhonghua2003@163.com |
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| Abstract
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| [Objective] To establish a HPLC method for simulataneous determination of Notoginsenoside R1 (1), Ginsenoside Rg1 (2), lobetyolin (3), Ginsenoside Rb1 (4), Ginsenoside Rd (5) in Wenxin Keli by HPLC.[Methods] The HPLC was conducted on Amethyst C18 column (4.6 mm×250 mm, 4 μm) with acetonitrile-water solution as themobile phase. The detection wavelength was 210 nm, and flow rate was 1 mL/min.[Results] Linearity of each standard was established within the concentration range of 8~247 mg/L for 1, 14~422 mg/L for 2, 1.5~42 mg/L for 3, 26.5~847 mg/L for 4 and 8~255 mg/L for 5. The average recovery(n=6) of the compound 1-5 was 102.69% with RSD of 2.74%, 98.72% with RSD of 1.85%, 97.69% with RSD of 1.94%, 102.11% with RSD of 1.96%, 99.66% with RSD of 2.49% respectively.[Conclusion] The treatment of samples was stable and reliable. The method can be used for determination of notoginsenoside R1, lobetyolin, ginsenoside Rg1, Rb1 and Rd in Wenxin Keli. |
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