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| Effect of scutellarin on the secretion of inflammatory mediators of RAW264.7 cell induced by LPS |
| Hits 2497 Download times 1690 Received:April 26, 2016 |
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| DOI
10.11656/j.issn.1672-1519.2016.08.11 |
| Key Words
scutellarin;inflammation;RAW264.7 cell;mechanism |
| Author Name | Affiliation | E-mail | | WEI Jing | Tianjin Modern Chinese Medicine Key Laboratory, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | CHEN Jing-rui | Tianjin Modern Chinese Medicine Key Laboratory, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | MIAO Lin | Tianjin Modern Chinese Medicine Key Laboratory, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | WANG Ya-dong | Tianjin Modern Chinese Medicine Key Laboratory, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | FAN Guan-wei | Tianjin Modern Chinese Medicine Key Laboratory, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | fgw1005@hotmail.com |
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| Abstract
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| [Objective] To study the effect of scutellarin on RAW264.7 cell inflammation induced by LPS, and to explore the mechanism of its anti-inflammatory effect. [Methods] To detect effect of scutellarin on RAW264.7 cell viability by MTT, detect effect of scutellarin on NF-κB transcription activity induced by LPS, detect scutellarin on TNF-α, IL-1β, IL-6 mRNA expression of NF-κB target gene in RAW264.7 cell induced by LPS. [Results] It had no obvious significance on RAW264.7 by 0.01～10 μmol/L scutellarin treatment for 24 h; 1, 10 μmol/L scutellarin could significantly inhibit NO generation and NF-κB transcription activity induced by LPS; scutellarin could inhibit up-regulation of TNF-α, IL-1β, IL-6 mRNA of NF-κB target gene in RAW264.7 cell induced by LPS. [Conclusion] Scutellarin has certain anti-inflammatory effect by inhibition NF-κB activation and inflammatory factor expression. |
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