|
| Effects of Salvianolate on neonatal rat cadiocytes apoptosis induced by H2O2 |
| Hits 2014 Download times 1457 Received:April 28, 2016 |
| View Full Text View/Add Comment Download reader |
| DOI
10.11656/j.issn.1672-1519.2016.08.12 |
| Key Words
Salvianolate;H2O2;cardiomyocyte;apoptosis;effect |
| Author Name | Affiliation | | LI Bing | Department of Emergency, Handan Central Hospital, Handan 056001, China |
|
| Abstract
|
| [Objective] To investigate the effects of Salvianolate on neonatal rat cadiocytes apoptosis induced by H2O2. [Methods] Cadiocytes of neonate rat was cultivated for 72 hours and divided into six groups:normal control group, H2O2 group, Salvianolate (50, 100, 200 mg/L)+H2O2 groups(n=8). The 12 hours after the drugs were given. The content of AST, CPK, LDH in culture medium were detected; the survival rate was detected by MTT; the cardiomyocytes apoptosis was observed by flow cytometry and the apoptosis rate was calculated; the expression of AKT mRNA, bcl-2 mRNA, Bax mRNA were detected by RT-PCR and the ratio of bcl-2/Bax was calculated; the expression of caspase-3, NF-κB protein was detected by Western blot and Semi-quantitative analysized; the activity of SOD, CAT and the content of MDA in cardiomyocytes were determinted. [Results] Compared with the H2O2 group, the content of AST, CPK, LDH in culture medium of Salvianolate (100, 200 mg/L)+H2O2 groups were significantly decreased; the survival rate were significantly increased; the cardiomyocytes apoptosis were improved and the apoptosis rate were significantly decreased; the expression of AKT, bcl-2 mRNA were significantly up-regulated, while the expression of Bax mRNA was significantly down-regulated, the ratio of bcl-2/Bax were significantly increased; the expression of caspase-3, NF-κB protein were significantly down-regulated; the activity of SOD, CAT in cardiomyocytes were significantly increased and the content of MDA was significantly decreased, all of the difference above were significant (P<0.05 or P<0.01). [Conclusion] Salvianolate had inhibitive effects on neonatal rat cadiocytes apoptosis induced by H2O2. |
|
|
|
|
|
|
|
