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| The establishment of UPLC-Digitized fingerprinting of multiple dosage forms of Xuefu Zhuyu decoction |
| Hits 1558 Download times 1625 Received:March 30, 2016 |
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| DOI
10.11656/j.issn.1672-1519.2016.09.13 |
| Key Words
Xuefu Zhuyu decoction;UPLC-digitized fingerprinting;principal component analysis |
| Author Name | Affiliation | E-mail | | YANG Long | Tianjin Modern Chinese Medicine Key Laboratory-Province and Ministry Co-established State Key Laboratory Cultivation Base, Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Research and Development Center of Traditional Chinese Medicine, Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin 300457, China | | | YANG Fan | Tianjin Modern Chinese Medicine Key Laboratory-Province and Ministry Co-established State Key Laboratory Cultivation Base, Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Research and Development Center of Traditional Chinese Medicine, Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin 300457, China | | | YANG Xin-ying | Tianjin Hongrentang Pharmaceutical Co., Ltd., Tianjin 300385, China | | | ZHANG Lei | Tianjin Modern Chinese Medicine Key Laboratory-Province and Ministry Co-established State Key Laboratory Cultivation Base, Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Research and Development Center of Traditional Chinese Medicine, Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin 300457, China | | | YU Hui-juan | Tianjin Modern Chinese Medicine Key Laboratory-Province and Ministry Co-established State Key Laboratory Cultivation Base, Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Research and Development Center of Traditional Chinese Medicine, Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin 300457, China | | | WANG Yue-fei | Tianjin Modern Chinese Medicine Key Laboratory-Province and Ministry Co-established State Key Laboratory Cultivation Base, Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Research and Development Center of Traditional Chinese Medicine, Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin 300457, China | wangyuefei_2006@hotmail.com |
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| Abstract
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| [Objective] To establish a sensitive and specific UPLC-digitized fingerprinting of multiple dosage forms of Xuefu Zhuyu decoction (capsule, oral liquid, tablet, soft capsule and Decoction) with UPLC-DAD technology. [Methods] The UPLC method was established by employing an ACQUITY UPLC® C18 (2.1×100 mm, 1.7 μm) with 0.1% formic acid aqueous solution (A)-acetonitrile (B) as mobile phase in a gradient elution (0~8 min, 3%~8% B; 8~24 min, 8%~19% B; 24~35 min, 19%~45% B). The detection wavelength was 280 nm. The flow rate was 0.3 mL/min and the column temperature was 50℃. [Results] The established method was employed to develop UPLC-digitized fingerprinting (UPLC-DFP) of multiple dosage forms of Xuefu Zhuyu decoction. By choosing rhoifolin as reference, 30 common peaks were obtained. 16 of 30 common peaks of Xuefu Zhuyu decoction were identified by comparing with standards. The similarity of the multiple dosage forms of Xuefu Zhuyu decoction is over 0.81, which was evaluated by Similarity Evaluation System for Chromatographic Fingerprint of TCM (Version 2004A). The relative peak area of common peaks as characteristic value was implemented to perform principal component analysis (PCA). The multiple dosage forms of Xuefu Zhuyu decoction were classified as three clusters based on different quality. [Conclusion] This method was simple, and provided the basis for the quality control of multiple dosage forms of Xuefu Zhuyu decoction. |
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