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| Optimization the purification progress on mulberry alkaloids based on the dissociation constants |
| Hits 1956 Download times 1876 Received:May 25, 2016 |
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| DOI
10.11656/j.issn.1672-1519.2016.10.11 |
| Key Words
mulberry alkaloids;1-deoxynojirimycin;cation exchange resin;dissociation constant;selective adsorption |
| Author Name | Affiliation | E-mail | | NIU An-song | Chinese Academy of Medical Sciences, State Key Laboratory of Bioactive Substances and Function of Natural Medicines, Beijing 100050, China
Chinese Academy of Medical Sciences, Beijing City Key Laboratory of Drug Delivery Technology and Novel Formulations, Beijing 100050, China | | | LIU Zhi-hua | Chinese Academy of Medical Sciences, State Key Laboratory of Bioactive Substances and Function of Natural Medicines, Beijing 100050, China
Chinese Academy of Medical Sciences, Beijing City Key Laboratory of Drug Delivery Technology and Novel Formulations, Beijing 100050, China | | | JIN Yi-qun | Beijing Wehand-Bio Pharmaceutical Co. LTD., Beijing 102600, China | | | ZHU Xiang-yang | Beijing Wehand-Bio Pharmaceutical Co. LTD., Beijing 102600, China | | | LIU Yu-ling | Chinese Academy of Medical Sciences, State Key Laboratory of Bioactive Substances and Function of Natural Medicines, Beijing 100050, China
Chinese Academy of Medical Sciences, Beijing City Key Laboratory of Drug Delivery Technology and Novel Formulations, Beijing 100050, China | ylliu@imm.ac.cn |
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| Abstract
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| [Objective] To select optimum regenerating agent of ion-exchange resin and optimize purification of mulberry alkaloids based on the pKa of the alkaloids and the pI of amino acids. [Methods] The 732 resins were regenerated with hydrochloric acid and different pH buffer solution, respectively. The productions were determined by RP-HPLC with pre-column derivatization. This article calculated the leakage amount of alkaloids and amino acids the adsorbing amount. Meanwhile it also determined the peak ratio of alkaloids in products. [Results] Ultimately, the total amino acids leaked in a short time with the buffer solution at pH 4.6 as regenerating agent. However, both of the alkaloids and amino acids didn't leak with hydrochloric acid as regenerating agent. The adsorption amount was increased from 1.84 to 2.23 mg/g with the buffer solution at pH 4.6 compared to pH 5.6. The relative peak ratio of the alkaloids in productions is 79.6% with the buffer solution at pH 5.6 as regenerating agent, and 23.6% with hydrochloric acid as regenerating agent. [Conclusion] Through adjusting the pH of resin separation and controlling the dissociation degree of the alkaloids and the amino acids, the purity of the production was improved with decreased adsorption of amino acids and increased selectivity of the resin. |
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