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| Anti-apoptotic and uterotrophic effects of Heyan Kuntai capsules in uterus of ovariectomized rats |
| Hits 2099 Download times 1785 Received:April 21, 2016 |
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| DOI
10.11656/j.issn.1672-1519.2016.11.11 |
| Key Words
Heyan Kuntai capsule;uterus morphology;apoptosis;Bcl-2 mRNA and protein;Bax mRNA and protein |
| Author Name | Affiliation | E-mail | | HUANG Chun-li | Traditional Chinese Medicine Research Institute of Tianjin University of Triditional Chinese Medicine, Tianjin 300193, China | | | ZHANG De-qin | Traditional Chinese Medicine Research Institute of Tianjin University of Triditional Chinese Medicine, Tianjin 300193, China | deqin123@163.com | | ZHANG Qing-xia | Traditional Chinese Medicine Research Institute of Tianjin University of Triditional Chinese Medicine, Tianjin 300193, China | | | WEN Jing | Traditional Chinese Medicine Research Institute of Tianjin University of Triditional Chinese Medicine, Tianjin 300193, China | |
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| Abstract
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| [Objective] To investigate the effects of Heyan Kuntai capsules on uterus morphology and apoptosis in ovariectomized rats.[Methods] Female SD rats underwent ovariectomize surgery and were divided into model group, progynova group, Remifemin group and Heyan Kuntai capsule of high, medium and low dose groups (8, 4 and 2 g crude drug/kg respectively). The drugs were administered for 8 weeks. This article observed the impacts of drugs on uterus pathology, staining and apoptosis, detected the expression levels of Bcl-2 and Bax mRNAs and proteins.[Results] It was obtained significantly decreased epithelial thickness and reduced myometrium and endometrium cross-sectional area and wall thickness in the model group compared with the control and Heyan Kuntai capsule treated groups(P<0.01 or P<0.05). It was also decreased Bcl-2 and increased Bax mRNAs and proteins expressions(P<0.01 or P<0.05) in the model group. Further more, the model group showed increased apoptosis rate(P<0.01 or P<0.05) and reduced number of glands(P<0.01 or P<0.05) in the uterus.[Conclusion] Heyan Kuntai capsules elicits uterotrophic effect and attenuates apoptosis in the uterus cells by regulating mRNAs and protein expressions of Bcl-2 and Bax. |
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